Ox-LDL诱导肝硬化患者巨噬细胞产生ET  

Ox-LDL-induced ETs production of macrophages in patients with cirrhosis

作　　者：孟庆洋[1] 徐春林[1] 康鹏[1] MENG Qing-yang;XU Chun-lin;KANG Peng(Department of Infectious Disease,the Second Affiliated Hospital of Harbin Medical University,Harbin 150086,China)

机构地区：[1]哈尔滨医科大学附属第二医院感染科

出　　处：《肝脏》2019年第9期1007-1010,共4页Chinese Hepatology

摘　　要：目的探讨氧化低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)诱导肝硬化患者巨噬细胞形成胞外诱捕网(ET)对肝硬化发展的作用。方法运用肝脏超声及肝脏弹性成像检测筛选肝硬化患者;通过ox-LDL等不同物质刺激巨噬细胞,观察ET的形成;检测ox-LDL、ET水平;诱导巨噬细胞表型转换。结果Ox-LDL刺激健康人巨噬细胞无ET产生;Ox-LDL可诱导肝硬化患者巨噬细胞形成ET,且在刺激后5 h达到峰值;ET诱导巨噬细胞表型M1向表型M2转化。结论ox-LDL可诱导肝硬化患者巨噬细胞产生ET,可能与肝硬化发展机制有关。Objective To investigate whether the oxidized low-density lipoprotein(ox-LDL)in cirrhosis patients induces macrophages to release extracellular traps(ETs)and promotes the development of cirrhosis.Methods Liver ultrasound and transient elastography were used to screen patients with cirrhosis.The formation of ETs was observed by stimulating macrophages with ox-LDL and other substances.Ox-LDL and ETs levels were detected.Phenotype transformation of macrophages was induced.Results Macrophages in healthy people did not release ETs with the stimulation of ox-LDL.Conversely,the ETs formation of macrophages in patients with cirrhosis was induced by the stimulation of ox-LDL,which reached its peak at 5 hours after stimulation.ETs induced the transformation of macrophages from phenotype M1 to M2.Conclusion Ox-LDL can induce macrophages to produce ETs in patients with cirrhosis,which may be related to the development mechanism of cirrhosis.

关 键 词：细胞外诱捕网 巨噬细胞 肝硬化 氧化低密度脂蛋白 

分 类 号：R54[医药卫生—心血管疾病]