姜黄素类似物L6H3对帕金森病大鼠脑部纹状体中单胺类神经递质代谢物的影响  

The effect of curcumin analogue L6H3 on striatum monoamine neurotransmitter in the rat model of Parkinson disease

作　　者：邹瑜驰 陈珺 黄文婷 刘欢 胡健 刘斐 王冬雪 朱丽云 林丽 ZOU Yuchi;CHEN Jun;HUANG Wenting;LIU Huan;HU Jian;LIU Fei;WANG Dongxue;ZHU Liyun;LIN Li(Department of Geriatric Medicine,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;School of Pharmaceutical Sciences,Wenzhou Medical University,Wenzhou 325035,China)

机构地区：[1]温州医科大学附属第一医院老年医学科,浙江温州325015 [2]温州医科大学药学院,浙江温州325035

出　　处：《温州医科大学学报》2019年第9期625-629,共5页Journal of Wenzhou Medical University

基　　金：国家自然科学基金资助项目(81771284);温州市科技计划项目(Y20170069)

摘　　要：目的:研究姜黄素类似物L6H3对帕金森病(PD)大鼠脑纹状体中7种单胺类神经递质及其代谢物的影响。方法:采用脑部纹状体定位注射6-羟基多巴胺(6-OHDA)建立PD大鼠模型,将大鼠随机分为假手术组、PD组和PD+L6H3组。通过微透析活体取样技术和高效液相色谱电化学检测法(HPLC-ECD)联用测定PD组大鼠脑纹状体细胞外液中单胺类神经递质及其代谢物含量。结果:造模后第6周(即给药第2周)及造模后第7周(即停药第1周),PD+L6H3组大鼠脑纹状体的多巴胺(DA)、3,4-二羟基苯乙酸(DOPAC)、高香草酸(HVA)、去甲肾上腺素(NE)、肾上腺素(E)、5-羟色胺(5-HT)、5-羟基吲哚乙酸(5-HIAA)与PD组比较明显升高(P<0.01)。结论:L6H3在给药后第2周及停药后1周均能明显提高PD大鼠脑中单胺类神经递质及其代谢物的含量。Objective:To investigate the effect of curcumin analogue L6H3 on seven monoamine neurotransmitters and metabolite in the striatum of rats with Parkinson disease(PD)by micro-dialysis sampling coupled with high performance liquid chromatography and electrochemical detection(HPLC-ECD).Methods:PD model was established by injecting 6-OHDA into the right striatum of rats,then verified by the rotational behavior after intraperitoneal injection of apomorphine solution.Rats were randomly divided to Sham group,PD group and PD+L6H3 group.The microdialysis sampling coupled HPLC-ECD was developed and applied to detect the level of monoamine neurotransmitters and metabolite in rat striatum.Results:6 weeks(2 weeks after administration),7 weeks(1 week after stopping administration)after model establishment,the levels of DA,DOPAC,HVA,NE,E,5-HT,5-HTAA in PD+L6H3 group were significantly increased compared with PD group(P<0.01).Conclusion:L6H3 has the capability to up-regulate monoamine neurotransmitters and metabolite in the striatum of PD rat model at both 2 weeks after administration and 1 week after stopping administration.

关 键 词：帕金森病 脑部纹状体 姜黄素类似物 高效液相色谱-电化学检测法 单胺类神经递质 大鼠 

分 类 号：R964[医药卫生—药理学]