不同浓度交泰丸及其组成药物对小鼠镇静作用的比较  

Sedative effect of Jiao-Tai-Wan with different concentrations and compositions on mice

作　　者：倪小芬[1] 林思思[1] 陈钱 袁拯忠[1] NI Xiaofen;Lin Sisi;CHEN Qian;YUAN Zhengzhong(Department of Traditional Chinese Medicine,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325015,China;Wenzhou Medical University,Wenzhou 325035,China)

机构地区：[1]温州医科大学附属第一医院中医科

出　　处：《温州医科大学学报》2019年第9期639-643,648,共6页Journal of Wenzhou Medical University

基　　金：国家自然科学基金资助项目(81673733)

摘　　要：目的:比较不同浓度交泰丸和黄连、肉桂对小鼠的镇静作用。方法:交泰丸配置成高、中、低浓度组,浓度分别为0.220 g/mL,0.110 g/mL和0.055 g/mL;黄连配置成高、中、低浓度组,浓度分别为0.200、0.100、0.050 g/mL;肉桂组浓度为0.030 g/mL;唑吡坦组(0.200 mg/mL)为阳性对照组,注射用水为空白对照组;分别灌胃小鼠7 d。用自发活动仪,连续监测给药7 d及停药1 d的自主活动次数,选取给药第1、第3、第5、第7天和停药1 d,计算并比较小鼠19:00给药后或停药后同时段15 min、30 min、1 h、3 h、12 h以及24 h的自主活动次数。结果:与空白对照组比较,给药第1、第3、第5、第7天及停药1 d后,交泰丸高浓度组给药后或停药后同时段30 min、1 h、3 h、12 h、24 h自主活动次数均减少,差异均有统计学意义(P<0.05);但是给药后15 min自主活动无显著性减少。与空白对照组比较,给药第3、第5、第7天,交泰丸中浓度组在1 h自主活动次数均减少,差异均有统计学意义(P<0.05),给药第7天,交泰丸低浓度组在1 h自主活动次数减少,差异有统计学意义(P<0.05)。与空白对照组比较,给药第3、第5、第7天,黄连高浓度组、中浓度组15 min、1 h自主活动次数均减少,差异均有统计学意义(P<0.05);但是给药后30 min黄连高浓度组、中浓度组自主活动无显著性减少(P>0.05);给药第7天,黄连高浓度组3 h、12 h、24 h自主活动次数均减少,差异均有统计学意义(P<0.05)。结论:交泰丸有镇静药效,其中黄连发挥主要作用。Objective:To compare sedative effects of Jiao-Tai-Wan with separated prescriptions of Coptis and Cinnamon on mice.Methods:Jiao-Tai-Wan groups were intragastric administrated 7 days by Jiao-Tai-Wan respectively with high,medium and low dosage of 0.220,0.110 and 0.055 g/mL.Coptis groups were respectively administrated 7 days by Coptis with high,medium and low dosage of 0.200,0.100 and 0.050 g/mL.Cinnamon high dose group was administrated 7 days by Cinnamon with concentration of 0.030 g/mL.Positive control group was administrated by Zolpidem with concentration of 0.200 g/mL.Blank control group was injected with water.The locomotor activity meter was used to determine the total number of locomotor activities of mice within 15 min,30 min,1 h,3 h,12 h,24 h after administration from day 1 to day 7.The number of locomotor activities of mice on day 8 without administration continued to be monitored.Results:On day 1 within 30 min,1 h,3 h,12 h,24 h after administration,the number of locomotor activities of Jiao-Tai-Wan group with high dose was statistically declined respectively compared with blank control group(P<0.05).These differences were observed again on day 3,day 5,day 7.These differences remained on day 8 without administration.On day 3,day 5,day 7 within 1 h,the number of locomotor activities of Jiao-Tai-Wan group with medium dose were statistically declined respectively compared with blank control group(P<0.05).On day 7 within 1 h,the number of locomotor activities of Jiao-Tai-Wan group with low dose was statistically declined comparing with blank control group(P<0.05).On day 3,day 5,day 7 within 15 min and 1 h,the frequency of locomotor activity of Coptis groups with high dosage and medium dosage were statistically declined respectively compared with blank control group(P<0.05).On day 7 within 3 h,12 h and 24 h after drug administration,the frequency of locomotor activity of Coptis group with high dosage was statistically declined c respectively omparing with blank control group(P<0.05).Conclusion:Jiao-Tai-Wan ca

关 键 词：交泰丸 镇静 自主活动 黄连 肉桂 小鼠 

分 类 号：R289[医药卫生—方剂学]