高灵敏度HBV DNA临床检测应用现状及发展趋势  

Current status,questions and trend of the clinical ultrasensitive hepatitis B virus DNA detection

作　　者：里进[1] 李一荣[1] LI Jin;LI Yirong(Department of Laboratory Medicine,zhongnan hospital,wuhan university,Wuhan,Hubei,China,430071)

机构地区：[1]武汉大学中南医院检验科，湖北 武汉 430071

出　　处：《分子诊断与治疗杂志》2019年第5期422-427,共6页Journal of Molecular Diagnostics and Therapy

基　　金：国家(十三五)科技重大专项课题“湖北及周边省传染病病原谱和流行规律研究”(2017ZX10103005)

摘　　要：乙型肝炎病毒(HBV)感染是一个严重的全球性公共健康问题,相比于传统的HBV DNA检测方法,高灵敏度HBV DNA技术因其检测下限低和线性范围广等优点,近年来受到临床医生的更多关注,许多专家和指南也推荐高灵敏度HBV DNA检测结果作为乙肝病毒核苷酸类药物治疗终点的监测指标。目前,国内外高灵敏度HBV DNA检测试剂种类较多,虽然原理基本类似,但检测效能却不尽相同,且在临床应用的过程中存在一些问题和局限性,本文就目前高灵敏度HBV DNA临床应用的现状做一分析总结,这有助于临床医生和检验同仁们更好地了解高灵敏度HBV DNA检测技术自身的特点,使该技术更好、更规范地应用于临床。Hepatitis B virus(HBV)infection is a serious global public health problem.At pres?ent,the ultrasensitive HBV DNA detection has increasingly prominent value in clinical diagnosis and decision of hepatitis B.Compared with traditional HBV DNA detection,more clinicians pay attention on the ultrasensi?tive HBV DNA detection owing to its low detection limit and wide linear range.Many experts and guidelines also recommend ultrasensitive HBV DNA detection as an important indicator of the end treatment?point of anti?HBV nucleotide drugs.At present,there are many kinds of ultrasensitive HBV DNA reagents at home and abroad.Although the principle is basically similar,the detection efficiency is different.Besides,there are many confusions in the process of clinical application.This paper summarizes the current status of clinical ap?plication of the ultrasensitive HBV DNA detection,which helps clinicians and laboratory colleagues to better understand the ultrasensitive HBV DNA detection technology.

关 键 词：乙型肝炎病毒 聚合酶链式反应 高灵敏度 质量控制 

分 类 号：R51[医药卫生—内科学]