Isolation and characterization of Bordetella pseudohinzii in mice in China  

作　　者：Lei Ma Shuwu Huang Yinzhu Luo Fangui Min Lifang He Meiling Chen Jinchun Pan Yu Zhang Jing Wang 

机构地区：[1]Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals Monitoring Institute

出　　处：《Animal Models and Experimental Medicine》2019年第3期217-221,共5页动物模型与实验医学（英文）

基　　金：Guangzhou Municipal Science and Technology Project, Grant/Award Number: 201904010284;Guangdong Provincial Science and Technology Project,Grant/Award Number: 2018B070714012

摘　　要：We report on the first detection and isolation of B.pseudohinzii(Bordetella pseudohinzii)in laboratory mice in China.Forty‐one B.pseudohinzii strains were isolated from 3094 mice in 33 different laboratory animal facilities in southern China.The isolates were identified through culture and genome sequenceing.Phylogenetic analysis based on the sequences of 16S rRNA and OmpA genes demonstrated that these strains were on the same clade as other B.pseudohinzii strains isolated from mice.Experimental infected mice presented an asymptomatic infection.B.pseudohinzii replicated in both the respiratory tract and the digestive tract.Most importantly B.pseudohinzii shed via feces and infected a group of sentinel mice in a separate cage via cage padding contaminated with B.pseudohinzii‐positive feces,indicating that B.pseudohinzii could transmit efficiently among mice and contaminate environmental facilities.Our study highlights the importance of routine monitoring of the pathogen in laboratory mice and provides vital insights into the transmission of Brodetellae in rodents and human.We report on the first detection and isolation of B. pseudohinzii(Bordetella pseudohinzii) in laboratory mice in China. Forty-one B. pseudohinzii strains were isolated from 3094 mice in 33 different laboratory animal facilities in southern China. The isolates were identified through culture and genome sequenceing. Phylogenetic analysis based on the sequences of 16 S r RNA and Omp A genes demonstrated that these strains were on the same clade as other B. pseudohinzii strains isolated from mice. Experimental infected mice presented an asymptomatic infection. B. pseudohinzii replicated in both the respiratory tract and the digestive tract. Most importantly B. pseudohinzii shed via feces and infected a group of sentinel mice in a separate cage via cage padding contaminated with B. pseudohinzii-positive feces, indicating that B. pseudohinzii could transmit efficiently among mice and contaminate environmental facilities. Our study highlights the importance of routine monitoring of the pathogen in laboratory mice and provides vital insights into the transmission of Brodetellae in rodents and human.

关 键 词：BORDETELLA pseudohinzii ISOLATION LABORATORY MICE TRANSMISSION 

分 类 号：R68[医药卫生—骨科学]