浆细胞性乳腺炎患者外周血Treg细胞及Treg/Th17比率的检测及意义  

Changes of Treg cells and Treg/Th17 raito in peripheral blood from the patients with plasma cell mastitis

作　　者：张少波[1] 黄惠玲 郑晓宏[1] 刘琦[1] 张诚华[1] ZHAO Shao-bo;HUANG Hui-ling;ZHENG Xiao-hong;LIU Qu;ZHANG Cheng-hua(Department of General Surgery,180th Hospital of People's Liberation Army,Quanzhou 362000,China)

机构地区：[1]中国人民解放军第180医院普外科

出　　处：《中国现代普通外科进展》2019年第8期611-614,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨浆细胞性乳腺炎(PCM)患者外周血Treg细胞及Treg/Th17比率的变化及意义。方法:采用流式细胞术检测18例急性期PCM患者(APCM)、32例亚急性期PCM患者(SPCM)、26例慢性期PCM患者(CPCM)和20例健康对照者(HC)外周血中Treg细胞水平;采用ELISA检测血浆中IL-10和TGF-β1水平。结果:APCM、SPCM和CPCM三组患者外周血Treg数量、Treg/Th17比率、IL-10和TGF-β1水平依次增高,但均低于HC组(P<0.05)。Treg/Th17比率与白细胞计数(WBC)呈负相关(r=-0.261,P=0.025),血浆TGF-β1水平与PTL水平呈负相关(r=-0.259,P=0.026)。结论:PCM患者外周血Treg细胞及相关细胞因子数量下降,导致Treg/Th17比率下降,表明Treg/Th17失衡在PCM发生发展及转归中具有重要作用。Objective:To observe the quantitative changes of Treg cells and Treg/Th17 ratio in patients with plasma cell mastitis(PCM).Methods:76 patients with PCM were divided into 3 groups:acute PCM group(APCM,n=18),subacute PCM group(SPCM,n=32),chronic PCM group(CPCM,n=26),and 20 healthy volunteers as contro(HC).The frequencies of Treg cells in peripheral blood were assessed by flow cytometric and the levels of IL-10 and TGF-β1 were measured by enzyme-linked immunosorbent assay.Results:The frequencies of Treg cells and the levels of IL-10 and TGF-β1 were in turn increased in the peripheral blood of APCM,SPCM and CPCM,but lower than that in HC. Also, the ratio of Treg/Th17 was negatively correlated with White blood count(WBC), and TGF-β1 levels were negatively correlated with platelet(PLT). Conclusion: Treg/Th17 dysfunction exists in patient with PCM, and it may play a potential role in the onset and development of PCM.

关 键 词：浆细胞性乳腺炎 TREG细胞 IL-10 TGF-Β1 

分 类 号：R659.8[医药卫生—外科学]