右美托咪定预处理对新生期吸入七氟醚大鼠青年及成年期海马神经元凋亡的影响  

Effects of dexmedetomidine preconditioning on apoptosis of hippocampal neurons in young and adult rats with sevoflurane inhalation during neonatal period

作　　者：梁小丽 张洁 刘程曦 张超 徐珊 张益 朱昭琼 LIANG Xiaoli1];ZHANG Jie;LIU Chengxi;ZHANG Chao;XU Shan;ZHANG Yi;ZHU Zhaoqiong(The Affiliated Hospital of Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]遵义医科大学附属医院,贵州遵义563000 [2]贵州省麻醉与器官保护基础研究重点实验室 [3]遵义医科大学麻醉医学院 [4]遵义医科大学第二附属医院

出　　处：《山东医药》2019年第23期23-26,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81360180,81660193);遵义医学院附属医院硕士科研启动基金资助项目[院字(2016)48号]

摘　　要：目的观察右美托咪定(Dex)预处理对新生期吸入七氟醚大鼠青年及成年期海马神经元凋亡的影响。方法60只新生雄性大鼠随机分为单纯七氟醚吸入组(七氟醚组)、Dex预处理组+七氟醚吸入(Dex预处理组)与空白对照组(空白对照组),每组20只。七氟醚组在出生后的第7、14、21天腹腔注射3 mL/kg生理盐水,此后吸入2.6%七氟醚4 h,Dex预处理组将20μg/kg右美托咪定溶于3 mL/kg生理盐水,于相同时间点腹腔注射后吸入2.6%七氟醚4 h,空白对照组腹腔给予3 mL/kg生理盐水后放入相同环境中吸入运载气体4 h。于青年期(37日龄)和成年期(97日龄)测定海马神经元凋亡情况以及海马中激活型Caspase-3蛋白表达。结果七氟醚组青年期和成年期海马凋亡神经元数量、激活型Caspase-3相对表达量均高于同期Dex预处理组和空白对照组(P均<0.01)。三组青年期与成年期海马凋亡神经元数量、激活型Caspase-3相对表达量组内比较差异均无统计学意义(P均>0.05)。结论Dex预处理可显著减少新生期吸入七氟醚大鼠青年期和成年期海马神经元凋亡,从而保护脑组织。Objective To observe the effects of dexmedetomidine(Dex)preconditioning on apoptosis of hippocampal neurons in young and adult rats with sevoflurane inhalation during neonatal period.Methods Sixty neonatal male rats were randomly divided into three group:the sevoflurane group(group S),preconditioning with dexmedetomidine and sevoflurane group(group Dex),and control group(group C),with 20 rats in each group.Rats in the group S were intraperitoneally injected with 3 mL/kg saline before inhaling sevoflurane for 4 h on day 7,14 and 21;20μg/kg Dex dissolved in 3 mL/kg saline was injected intraperitoneally into animals in the group Dex before 4-hour sevoflurane inhalation at the same time points.Rats in the group C inhaled carrier gas for 4 h after injecting 3 mL/kg saline.The level of neuronal apoptosis and the expression of cleaved-Caspase-3 in hippocampus were investigated at juvenile(37 days old)and adult period(97 days old).Results At juvenile and adult stage,the number of apoptotic neurons and the expression of cleaved Caspase-3 in the group S were higher than those in the group C and group Dex(both P<0.01).Among the three group,there was no significant difference between juvenile stage and adult stage in terms of the number of apoptotic neurons and the expression of cleaved Caspase-3(all P>0.05).Conclusion Dex preconditioning can reduce the neuronal apoptosis induced by neonatal sevoflurane inhalation at the juvenile and adult stage,thereby protecting the brain tissues.

关 键 词：七氟醚 新生期 凋亡 海马神经元 学习记忆能力 大鼠 

分 类 号：R614.2[医药卫生—麻醉学]