子痫前期患者胎盘组织中水通道蛋白3的表达  

Expression of aquaporin 3 in placenta tissue of patients with preeclampsia

作　　者：张东方 王茜[3] 李治国 陈燕 ZHANG Dongfang;WANG Qian;LI Zhiguo;CHEN Yan(The Affiliated Hospital of North China University of Science and Technology,Tangshan,Hebei Province,063000;Kailuan General Hospital of Hebei Province;Tangshan Maternal and Child Health Care Hospital;North China University of Science and Technology)

机构地区：[1]华北理工大学附属医院,唐山063000 [2]河北省唐山市开滦总医院 [3]河北省唐山市妇幼保健院 [4]华北理工大学

出　　处：《中国计划生育学杂志》2019年第9期1155-1158,共4页Chinese Journal of Family Planning

基　　金：中国煤炭工业协会2018年度科学技术研究指导性计划项目(MTKJ2018-264)

摘　　要：目的:探讨水通道蛋白3(AQP3)在子痫前期患者胎盘组织中的表达及其意义。方法:选取2017年10月—2018年9月于本院住院的60例重度子痫前期患者为研究对象(子痫前期组),随机选取同期正常足月妊娠孕妇60例(正常妊娠组)为对照。采用免疫组化法测定AQP3在胎盘中定位,利用qRT PCR技术及Western blotting检测胎盘组织中AQP3 mRNA及其蛋白的表达。结果:免疫组化结果显示,AQP3在正常胎盘和子痫前期胎盘组织中均有表达,子痫前期胎盘组织中AQP3 mRNA相对表达量(0.81±0.70)低于正常妊娠组(1.45±0.18),AQP3蛋白的相对表达量(0.53±0.06)低于正常妊娠组(0.83±0.02)(均P<0.05)。结论:AQP3可能在先兆子痫的发病机制或适应性反应调节中起重要作用。Objective:To investigate the expression and significant of aquaporin 3(AQP3)in placenta tissue of patients with preeclampsia.Methods:60 patients with severe preeclampsia were selected in the study group from October 2017 to September 2018,and another 60 normal pregnant women were selected in the control group during the same time.The localization of AQP3 in placenta tissue of patients in both groups was detected by immunohistochemistry.The levels of mRNA of AQP3 and AQP3 of patients in both groups were measured by qRT PCR and western blot.Results:The results by immunohistochemistry showed that AQP3 localized in placenta tissue of patients in both groups,and the expression of AQP3 in placenta tissue of patients in the study group was significant lower than that of patients in the control group(P<0.05).The mRNA level of AQP3 of patients in the study group was 0.81±0.70,which was significant lower than that(1.45±0.18)of patients in the control group(P<0.05).The mRNA level of AQP3 of patients in the study group was 0.53±0.06,which was significant lower than that(0.83±0.02)of patients in the control group(P<0.05).Conclusion:AQP3 may play a crucial role either in pathogenesis or in the adaptative response regulation of preeclampsia.

关 键 词：子痫前期 胎盘 水通道蛋白3 

分 类 号：R71[医药卫生—妇产科学]