固相萃取与毛细管电泳在线联用电渗驱动液流阀接口的设计与评价  

Design and Evaluation of a Sheath Flow Gating Interface for the On-line Coupling of Solid-phase Extraction with Capillary Electrophoresis

作　　者：李金祥[1] 马世雍 李佳[1] 杨芮萌 LI Jin-xiang;MA Shi-yong;LI Jia;YANG Rui-meng(School of Chemistry and Chemical Engineering,Liaoning Normal University,Dalian Liaoning 116029,China)

机构地区：[1]辽宁师范大学化学化工学院

出　　处：《德州学院学报》2019年第4期16-19,共4页Journal of Dezhou University

摘　　要：本实验自主搭建了固相萃取(SPE)和毛细管电泳(CE)的在线联用系统.设计了一种采用电渗驱动鞘流式液流阀新型接口,背景缓冲液对SPE毛细管出口端的冲洗由以往的压力驱动改进为电渗驱动.两个毛细管间距离为320μm,其死体积为15.7 nL.背景缓冲液为10 mM的Na2HPO4,允许的最大冲洗电压为5.0 kv,最大样品流速为2.0μL min^-1.在5小时内考察了取样的重现性,峰高、峰面积和迁移时间的相对标准偏差平均值分别为4.1%、1.6%、0.5%,表明该接口具有很好的稳定性.In this work,an on-line coupling system of solid-phase extraction(SPE)with capillary electrophoresis(CE)has been developed by using a home-made electroosmotic drive sheath flow gating interface.The washing of SPE capillary outlet by background buffer was changed from pressure drive to electroosmotic drive.The distance between two capillary tubes is 320μm,and the dead volume is 15.7 nL.The maximum rising voltage allowed for 10 mM Na2HPO4 background buffer is 5.0 kV and the maximum sample flow rate is 2.0μL?min^-1.The relative standard deviations obtained through consecutive run over 5.0 h period were 4.1%,1.6%and 0.5%,respectively.These RSD values shown that the interface has a good stability.

关 键 词：固相萃取 毛细管电泳 接口 

分 类 号：O657.7[理学—分析化学]