一测多评法测定沉香化气片中9种活性成分的含量  

Determination of nine effective constituents in Chenxiang-Huaqi tablets by quantitative analysis of multi-components by single marker

作　　者：王温才 金蕾 张贵萍[2] Wang Wencai;Jin Lei;Zhang Guiping(Pharmaceutical Clinical Trial Research Section of Qingdao Municipal Hospital,Qingdao 266000,China;Central Pharmacy of Pharmacy Department of Qingdao Municipal Hospital,Qingdao 266000,China)

机构地区：[1]山东省青岛市市立医院药物临床试验研究科,266000 [2]山东省青岛市市立医院药剂科中心药房,266000

出　　处：《国际中医中药杂志》2019年第9期986-991,共6页International Journal of Traditional Chinese Medicine

摘　　要：目的建立一测多评法测定沉香化气片中9种活性成分含量的方法.方法采用HPLC法,流动相为乙腈(A)-0.2%磷酸溶液(B),梯度洗脱;流速1.0 ml/min;甘草苷、甘草酸铵、木香烃内酯、去氢木香内酯检测波长230 nm,芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷检测波长283 nm,广藿香酮检测波长310 nm;柱温30℃;进样量10μl,以橙皮苷为内参物,建立一测多评法同时测定甘草苷、甘草酸铵、木香烃内酯、去氢木香内酯、芸香柚皮苷、柚皮苷、新橙皮苷和广藿香酮8种成分的相对校正因子并计算其含量,并与外标法分析比较以上9种成分的含量差异.结果一测多评法测得的橙皮苷、甘草苷、甘草酸铵、木香烃内酯、去氢木香内酯、芸香柚皮苷、柚皮苷、新橙皮苷和广藿香酮9种成分含量计算值与外标法测定值的差异无统计学意义(P>0.05).结论该方法可为建立沉香化气片的质量标准提供参考.Objective To develop a method of quantitative analysis of multi-components by single marker for nine effective constituents in Chenxiang-Huaqi tablets. Methods Using HPLC method, the mobile phase was acetonitrile -0.2% physcion acid in gradient elution program, the flow rate was 1.0 ml/min. The detection wavelength was set at 230 nm for liquiritin, ammonium glycyrrhizinate, costunolide and dehydrocostus lactone, and 283 nm for narirutin, naringin, hesperidin and neohesperidin, and 310 nm for pogostone. The column temperature was 30 and the injection volume was 10 μl.℃ Using Hesperidin as the internal standard, the relative correction factors among liquiritin, ammonium glycyrrhizinate, costunolide, dehydrocostus lactone, narirutin, naringin, neohesperidin, pogostone were detected by QAMS and their contents were calculated. The content difference of the above nine components with the external standard method were compared. Results The calculated values of hesperidin, liquiritin, ammonium glycyrrhizinate, costunolide, dehydrocostus lactone, narirutin, naringin, neohesperidin, pogostone were not statistical significance from those measured by external standard method (P>0.05). Conclusions The method offered reference for quality standard of Chenxiang-Huaqi tablets.

关 键 词：含量测定(中药) 沉香化气片 一测多评法 甘草苷 木香烃内酯 去氢木香内酯 芸香柚皮苷 柚皮苷 橙皮苷 新橙皮苷 广藿香酮 高效液相色谱法 

分 类 号：R28[医药卫生—中药学]