鸡矢藤颗粒剂干预大鼠肝纤维化模型TGF-β1/Smad通路试验  

Paederia scandens granules of intervention in rat liver fibrosis model experimental study on TGF-β1/Smad pathway

作　　者：袁勇[1,2] 吕传柱[3] YUAN Yong;LV Chuan-zhu(Hainan Medical University,Haikou,Hainan 570102,China;Key Laboratory of Emergency and Trauma of Ministry of Education,Hainan Medical University,Haikou,Hainan 570102,China;Department of Emergency,Clinical Research Center for Acute and Critical Diseases of Hainan Province,The Second Affiliated Hospital of Hainan Medical University,Haikou,Hainan 570102,China)

机构地区：[1]海南医学院,海南海口570102 [2]海南医学院急救与创伤研究教育部重点实验室,海南海口570102 [3]海南医学院第二附属医院海南省急危重症临床医学研究中心,海南海口570102

出　　处：《食品与机械》2019年第9期6-8,共3页Food and Machinery

基　　金：2014年海南省自然科学基金（编号：0104777039）;2012年海南省卫生厅科研项目（编号：琼卫2012PT-32）

摘　　要：将30只大鼠随机分为正常对照组(A组)、非鸡矢藤治疗模型组(B组)和鸡矢藤模型组(C组)。8周后荧光实时定量法检测TGF-β1、Smad3和Smad7的mRNA的表达。结果表明:从白介素-1β(IL-1β)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)酶联免疫数据看,C组与A组差异极显著(P<0.01),与B组差异显著(P<0.05);B组与A组差异极显著(P<0.01)。采用鸡矢藤治疗后,可以明显抑制TGF-β1 mRNA的表达,C组与A组差异显著(t=2.245,P<0.05),与B组差异极显著(t=1.142,P<0.01);采用鸡矢藤治疗后,可以明显抑制Smad3 mRNA的表达,C组与A组差异显著(t=2.149,P<0.05),与B组差异极显著(t=1.206,P<0.01);采用鸡矢藤治疗后,可以提升Smad7 mRNA的表达,C组与A组差异显著(t=2.201,P<0.05),与B组差异极显著(t=1.007,P<0.01)。鸡矢藤可以抑制TGF-β1 mRNA和Smad3 mRNA的表达,促进Smad7 mRNA的表达。研究表明,在肝纤维化的作用机制中,存在Smad3 mRNA-(TGF-β1 mRNA)-Smad7 mRNA的轴性作用通道。Thirty rats were randomly divided into normal control group(group A),non-paederia scandens treatment model group(group B)and paederia scandens treatment model group(group C).The expression of mRNA of TGF-β1,Smad3 and Smad7 was detected by real-time quantitative fluorescence method after 8 weeks.The results showed that,according to the ELISA data of interleukin-1β(IL-1β),interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α),the difference between group C and group A was extremely significant(P<0.01),which is the same as between group B and group A,and that the difference between group C and group B was significant(P<0.05).The difference between group B and group A was extremely significant(P<0.01).After treatment with paederia scandens,the expression of TGF-β1 mRNA was significantly inhibited,the difference between group C and group A was significant(t=2.245,P<0.05),and the difference between group C and group B was extremely significant(t=1.142,P<0.01).After treatment with Paederia scandens,the expression of Smad3 mRNA was significantly inhibited,the difference between group C and group A was significant(t=2.149,P<0.05),and the difference between group C and group B was extremely significant(t=1.206,P<0.01).Afterwards,the expression of Smad7 mRNA was increased,the difference between group C and group A was significant(t=2.201,P<0.05),and the difference was significant between group C and group B(t=1.007,P<0.01).Paederia scandens can inhibit the expression of TGF-β1 mRNA,decrease the expression of Smad3 mRNA,and increase the expression of Smad7 mRNA.It is shown in our study that in the mechanism of liver fibrosis,there exists a linear channel effect of Smad3 mRNA-(TGF-β1 mRNA)-Smad7 mRNA.

关 键 词：鸡矢藤 肝纤维化 转化生长因子 

分 类 号：R28[医药卫生—中药学]