基于碱裂解法川贝母的鉴别  

Study on identification of fritillariae cirrhosae bulbus by improved alkali pyrolysis

作　　者：陈玉秀 彭必武 李雨嫣 CHEN Yu-xiu;PENG Bi-wu;LI Yu-yan(Hunan Food and Drug Vocational College,Changsha,Hunan 410208,China)

机构地区：[1]湖南食品药品职业学院

出　　处：《食品与机械》2019年第9期94-97,共4页Food and Machinery

基　　金：科技部国家科技基础平台项目（编号：2005DK21400）;湖南省食品药品监督管理局食品药品安全科技项目（编号：湘食药科R2017010）

摘　　要：利用碱裂解法提取川贝母DNA,考察不同裂解液配比对川贝母DNA质量与电泳成功率的影响,并与现行方法相比较。结果表明,含有200 mmol/L NaCl溶液,5 mmol/L Mg 2 Cl溶液,1%Triton X-100,1%PVP,1%SDS,2 mmol/L EDTA,0.1 mol/L Tris-HCl配比液的碱裂解液具有最佳的DNA提取效果,此碱裂解液提取的DNA电泳条带与现行方法一致,可节省15~20 min,单个样品检验成本可降至0.01元。采用该方法对25个市售贝母样本进行鉴定,可快速鉴别出川贝母的真伪。The DNA of Fritillariae cirrhosae bulbus was extracted by alkali lysis.The effects of different proportion of lysate on DNA quality and electrophoretic success rate were investigated,which compared with the current methods.Results,The alkaline lysis buffer had the best effect on DNA extraction,which was made of 200 mmol/L NaCl,5 mmol/L Mg 2 Cl,1%Triton X-100,1%PVP,1%SDS,2 mmol/L EDTA and 0.1 mol/L Tris-HCl.The DNA electrophoretic bands extracted by this alkali lysate were in accordance with the current method.The method can save 15~20 minutes,and the cost of single sample testing can be reduced to 0.01 yuan.This method was used to identify 25 samples of Fritillariae cirrhosae bulbus on the market.The results showed that Fritillariae cirrhosae bulbus could be identified quickly.

关 键 词：川贝母 碱裂解法 DNA提取 快速鉴别 

分 类 号：R28[医药卫生—中药学]