子宫内膜异位症患者SDF-1、CXCR-4水平的变化及临床意义  

Changes of stromal cell derived factor-1 and chemokine receptor-4 levels in patients with endometriosis and their clinical significance

作　　者：王飞鹏 张晓英 姜晓春 WANG Fei-peng;ZHANG Xiao-ying;JIANG Xiao-chun(Department of Gynecology,the Central Hospital of Xianyang City,Xianyang 712000,Shaanxi,CHINA)

机构地区：[1]咸阳市中心医院妇科

出　　处：《海南医学》2019年第18期2379-2382,共4页Hainan Medical Journal

摘　　要：目的探讨子宫内膜异位症患者基质细胞衍生因子-1(SDF-1)、趋化因子受体-4(CXCR-4)水平的变化及其临床意义。方法收集咸阳市中心医院2017年10月至2018年10月收治的40例子宫内膜异位症患者的在位内膜(在位组)及异位内膜(异位组)组织标本,另取40例子宫肌瘤患者正常子宫内膜组织作为对照组,采用免疫组织化学和逆转录定量PCR(qRT-PCR)测定并比较各组患者的SDF-1、CXCR-4水平。结果SDF-1、CXCR-4在各组患者的腺上皮细胞和间质细胞中均有分布,其中腺上皮细胞有明显着色;异位组患者的SDF-1、CXCR-4平均光密度值分别为0.194±0.025、0.169±0.081,明显高于在位组的0.140±0.021、0.121±0.006和对照组的1.026±0.159、1.007±0.181,且在位组SDF-1、CXCR-4平均光密度值明显高于对照组,差异均有统计学意义(P<0.05);异位组患者的SDF-1、CXCR-4 mRNA的相对表达量分别为3.015±0.613、3.142±0.281,明显高于在位组的1.901±0.319、1.651±0.219和对照组的1.026±0.159、1.007±0.181,且在位组SDF-1、CXCR-4 mRNA的相对表达量明显高于对照组,差异均有统计学意义(P<0.05)。结论子宫内膜异位内膜组织、在位内膜组织中SDF-1、CXCR-4的表达均上调,可能参与子宫内膜异位症的发生发展。Objective To investigate the changes and clinical significance of stromal cell derived factor-1(SDF-1)and chemokine receptor-4(CXCR-4)levels in patients with endometriosis.Methods Forty specimens of eutopic endometrium(eutopic group)and ectopic endometrium(ectopic group)were collected from patients with endometriosis in the Central Hospital of Xianyang City from October 2017 to October 2018.Another 40 specimens of normal endometrium from patients with hysteromyoma were taken as control group.The levels of SDF-1 and CXCR-4 in each group were measured by immunohistochemistry and quantitative reverse transcription polymerase chain reaction(qRT-PCR).Results SDF-1 and CXCR-4 were distributed in glandular epithelial cells and stromal cells of all groups,and glandular epithelial cells were markedly stained.The average optical densities of SDF-1 and CXCR-4 in ectopic group were 0.194±0.025 and 0.169±0.081,respectively,which were significantly higher than 0.140±0.021 and 0.121±0.006 in eutopic group and 1.026±0.159 and 1.007±0.181 in control group(P<0.05),and the values were significantly higher in eutopic group than control group(P<0.05).The relative expression levels of SDF-1 and CXCR-4 mRNA in ectopic group were 3.015±0.613 and 3.142±0.281,respectively,which were significantly higher than 1.901±0.319 and 1.651±0.219 in eutopic group and 1.026±0.159 and 1.007±0.181 in control group(P<0.05),and the levels were significantly higher in eutopic group than control group(P<0.05).Conclusion The expression levels of SDF-1 and CXCR-4 in ectopic endometrium and eutopic endometrium were up-regulated,which may be involved in the occurrence and development of endometriosis.

关 键 词：子宫内膜异位症 趋化因子受体-4 基质细胞衍生因子-1 相对表达量 临床意义 

分 类 号：R711.74[医药卫生—妇产科学]