玳玳果黄酮降脂提取物4个效应组分的含量测定研究  

Study on the Content Determination of the Four Effective Components in Daidai Flavonoids Lipid-Lowering Extract

作　　者：马国萍[1] 陈丹[1] 朱仙慕 刘秀棉 洪丽婷 陈思 陈红[2] MA Guoping;CHEN Dan;ZHU Xianmu;LIU Xiumian;HONG Liting;CHEN Si;CHEN Hong(Department of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Institute of Senile Disease,Fujian Provincial Hospital Cadre Special Clinic,Fuzhou 350001,China)

机构地区：[1]福建中医药大学药学院,福州350122 [2]福建省立医院干部特诊科,临床老年病研究所,福州350001

出　　处：《福建医科大学学报》2019年第4期214-218,共5页Journal of Fujian Medical University

基　　金：福建省自然科学基金(2018J01253);福建省医学创新项目(2016-CX-45);福建省科技计划项目(2010Y2004)

摘　　要：目的建立HPLC法同时测定玳玳果黄酮降脂提取物4个效应组分新橙皮苷、柚皮苷、橙皮内酯水合物、枳属苷的含量。方法采用高效液相色谱法,色谱柱Lichrocart C18 柱(250 mm×4.6 mm,5 μm),以乙腈-0.2%醋酸水溶液为流动相;程序洗脱;检测波长284 nm;流速1.0 mL/min;柱温30 ℃;进样量10 μL。外标法同时测定提取物效应组分新橙皮苷、柚皮苷、橙皮内酯水合物、枳属苷的含量。结果玳玳果黄酮降脂提取物新橙皮苷、柚皮苷、橙皮内酯水合物、枳属苷的质量浓度分别在76.365~1 491.482 μg/mL(r=0.999 4),49.155~1 024.283 μg/mL(r=0.999 7),5.134~61.151 μg/mL(r=0.999 5),1.771~40.962 μg/mL(r=0.999 8)范围内呈良好的线性关系;平均回收率(n=6)分别为100.3%,102.2%,97.98%及95.16%;相对标准偏差分别为1.50%,1.79%,1.70%及2.42%。结论所建立的HPLC法同时测定提取物4个效应组分群含量的方法,可用于多指标定量监控玳玳果黄酮降脂提取物的质量。Objective To establish a HPLC method for the simultaneous determination of the four effective components(neopperidin,naringin,hesperidolide hydrate,and poncirin)in Daidai flavonoids lipidlowering extract.MethodsWe used high performance liquid chromatography and chromatographic column Lichrocart C18(250 mm×4.6 mm,5μm).Acetonitrile 0.2% acetic acid solution was used as the mobile phase.Gradient elution was used,with flow rate 1.0 mL/min,detection wavelength 284 nm,column temperature 30℃,and injection volume 10μL.External standard method was established for the simultaneous determination of the contents of neohesperidin,naringin,Hesperidin Hydrate,and poncirin in Daidai flavonoids lipidlowering extract.ResultsThe concentration of nehesperidin,naringin,hesperidate lactone hydrate,and poncirin were linear in the range of 76.3651 491.482μg/mL(r=0.999 4),49.1551 024283μg/mL(r=0.999 7),5.13461.151μg/mL(r=0.999 5),1.771-40.962μg/mL(r=0.999 8)respectively.The average recovery rates(n=6)were 100.3%,102.2%,97.98%,and 95.16%,respectively.The relative standard were 1.50%,1.79%,1.70%,2.42%,respectively.ConclusionThe established HPLC method for simultaneous determination of the four effective components in the extracts can be used to quantitatively monitor the quality of Daidai flavonoids lipidlowering extract.

关 键 词：黄酮 橙皮苷 陈皮 内酯类 色谱法 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]