镉逆境胁迫下甜菜BvHIPP24基因在大肠杆菌的功能分析  

Functional Analysis of Beta vulgaris BvHIPP24 in E. coli under Cadmium Stress

作　　者：鲁振强[1] 王锦霞 董大鹏 张司扬 刘大丽 Lu Zhenqiang;Wang Jinxia;Dong Dapeng;Zhang Siyang;Liu Dali(College of Life Sciences,Heilongjiang University,Harbin 150080;Key Laboratory of Sugar Beet Genetics and Breeding/Crop Science Institute of Heilongjiang University,Harbin 150080;College of Life Science and Technology,Harbin Normal University,Harbin 150025)

机构地区：[1]黑龙江大学生命科学学院,哈尔滨150080 [2]黑龙江省普通高等学校甜菜遗传育种重点实验室/黑龙江大学农作物研究院,哈尔滨150080 [3]哈尔滨师范大学生命科学与技术学院,哈尔滨150025

出　　处：《中国糖料》2019年第4期6-10,共5页Sugar Crops of China

基　　金：黑龙江省自然科学基金项目(LH2019C057);国家自然基金青年基金项目(31601229);农业部糖料现代产业技术体系建设项目(CARS-170204、CARS-170111)

摘　　要：重金属关联异戊二烯化植物蛋白(Heavy metal-associated isoprenylated plant proteins,HIPPs)由于其独特的重金属结合域(heavy metal associated domains,HMA)和异戊二烯序列(isoprenylation motif)的结构特点,使其成为一类重要的金属分子伴侣(Metallochaperones)。本研究利用RT-PCR的方法,从甜菜体内克隆获得了Beta vulgaris heavy metal-associated isoprenylated plant protein24(BvHIPP24)基因。该基因CDS全长为444 bp,编码了147个氨基酸,分子量为16.38 kDa。利用大肠杆菌表达载体构建pEASY-Blunt E1-BvHIPP24重组质粒,并转化到BL21中。通过1 mmol/L IPTG诱导发现:在0.5 mmol/L Cd2+逆境胁迫下,表达BvHIPP24重组菌的生长趋势要明显优于对照菌株,并具有更高的Cd耐受性。因此可以推断,甜菜BvHIPP24基因在镉逆境胁迫过程中发挥着重要的作用,并有可能参与到甜菜细胞重金属离子吸收、转运、区隔以及代谢平衡调控过程中。Because of unique heavy metal associated domains(HMA)and isoprenylation motif,heavy metalassociated isoprenylated plant proteins(HIPPs)are important members in Metallochaperones.In this study,Beta vulgaris heavy metal-associated isoprenylated plant protein24(BvHIPP24)was cloned by RT-PCR in sugar beet.Its full length of CDS was 444 bp,encoding 147 amino acids with a molecular weight of 16.38 kDa.The recombinant plasmid of pEASY-Blunt E1-BvHIPP24 was constructed and transformed into BL21.The results showed that,by 1 mmol/L IPTG induction,reconstructed E.coli expressing BvHIPP24 with superior Cd tolerance grew better than the control under 0.5 mmol/L CdCl2 stress.Hence,it could be concluded that BvHIPP24 played a vital role in cadmium stress process in sugar beet,and might be involved in cell heavy metal ion absorption,transportation,compartmentalization and metabolic homeostasis.

关 键 词：甜菜 重金属关联异戊二烯化植物蛋白 镉逆境 大肠杆菌 

分 类 号：S566.3[农业科学—作物学]