甘蔗梢腐病轮枝镰刀菌侵染甘蔗叶片的显微观察  

Microscopic Observation on the Infection of Sugarcane Leaves by Pokkah Boeng Pathogen Fusarium verticillioides

作　　者：刘丹丹 姚姿婷 赖小群 姚潇 张木清[2,3,4] 邹承武 陈保善[2,3,4] Liu Dandan;Yao Ziting;Lai Xiaoqun;Yao Xiao;Zhang Muqing;Zou Chengwu;Chen Baoshan(College of Life Science and Technology,Guangxi University,Nanning 530004;College of Agricultural,Guangxi University,Nanning 530004;State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources,Nannin 530004;Ministry of Education and Provincial Government Co-sponsored Cooperative Innovation Center for Sugarcane and Sugar Industry,Nanning 530004)

机构地区：[1]广西大学生命科学与技术学院,南宁530004 [2]广西大学农学院,南宁530004 [3]亚热带农业生物资源保护与利用国家重点实验室,南宁530004 [4]蔗糖产业省部共建协同创新中心,南宁530004

出　　处：《中国糖料》2019年第4期41-45,共5页Sugar Crops of China

基　　金：广西蔗糖产业协同创新中心项目(桂教科研[2014]13号);广西科技基地和人才专项(桂科AD17129002)资助

摘　　要：轮枝镰刀菌(Fusarium verticillioides)是引起甘蔗梢腐病的主要病原真菌之一。利用苯胺蓝染色法,对轮枝镰刀菌分生孢子在甘蔗叶片上的侵染过程进行显微观察,发现在28℃保湿条件下,24 h分生孢子形成芽管,48 h芽管发育为菌丝,从气孔或毛状体侵入叶片,之后菌丝在气孔下腔细胞处及毛状体细胞内增殖,并缠绕在气孔及毛状体周围,然后进一步侵染周边细胞;72 h菌丝从气孔往外伸出,96 h受侵染组织坏死。Fusarium verticillioides(abbreviated Fv)is one of the major pathogenic fungi causing pokkah boeng on sugarcane.The microscopic observation of Fv conidial infection on sugarcane leaves was carried out by staining with aniline blue.It was found that at 28℃,conidia formed bud tube at 24 h,hyphae was developed by bud tube at 48 h,invaded leaves from stomata or trichomes,and then proliferated in substomatal cavity cells and hairy body cells,and wound around the stomata and trichomes,then further spread to adjacent cells.The hyphae protruded out of the stomata at 72 h and the infected tissues of cane plant were necrotic at 96 h.

关 键 词：甘蔗梢腐病 轮枝镰刀菌 分生孢子 侵染过程 显微观察 

分 类 号：S435.661[农业科学—农业昆虫与害虫防治]