DNA辅助纳米金三角片的高效纯化与信息编码  

DNA-assisted High-efficient Purifying and Encoding of Gold Nanotriangles

作　　者：鲁爽[1,2] 方维娜 王丽华 柳华杰[4] Shuang Lu;Wei-na Fang;Li-hua Wang;Hua-jie Liu(Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800;University of Chinese Academy of Sciences,Beijing 100049;Institute of Molecular Medicine,Renji Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200127;School of Chemical Science and Engineering,Tongji University,Shanghai 200092)

机构地区：[1]中国科学院上海应用物理研究所,上海201800 [2]中国科学院大学,北京100049 [3]上海交通大学医学院附属仁济医院分子医学研究院,上海200127 [4]同济大学化学科学与工程学院,上海200092

出　　处：《高分子学报》2019年第9期964-972,I0004,共10页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号21722310,21873071)资助项目

摘　　要：针对目前纳米金三角片的分离纯化手段仍不够成熟这一问题,提出借助巯基修饰的DNA分子,对混有类球形颗粒等杂质的纳米金三角片粗产物,进行形状选择性分离纯化的新思路.纳米金表面修饰的DNA分子,一方面屏蔽了金与溶液环境的直接接触,提高了颗粒的稳定性;另一方面,形貌粒径以及表面DNA修饰量的差异,造成不同纳米金颗粒在电场中的迁移速度不同,因而可通过凝胶电泳手段分离纯化金颗粒.该方法获得的纳米金三角片纯度在80%以上,并对于不同粒径的三角片纯化具有普适性.更为重要的是,表面修饰的DNA同时实现了对纳米金三角片的信息编码,通过设计修饰DNA序列信息,可以实现后续的可控组装,构建更高级结构.Combining the advantages of information encoding and function realization,DNA-conjugated nanomaterials have attracted great attention recently.As a typical anisotropic material,gold nano-triangle is of great value due to the sharp vertices.However,the yield of nano-triange is not high through current wet synthesis.In order to solve this problem,we propose a new strategy of shape selective separation of gold nano-triangles with the assist of thiol-DNA.The attachment of DNA to the surface of gold nanoparticles,on one hand,can stabilize the nanoparticles by shielding the direct contact between gold nanoparticles and solution environment.On the other hand,according to the particle shape,size and the surface charge density,gold nanoparticles have different mobilities in the electric field.As a result,gold nanoparticles can be separated by gel electrophoresis.The DNAassist purification method gives a yield of above 80%of gold nano-triangles,which is also universal for the separation of nano-triangles with different sizes.More importantly,the surface-modified DNA can simultaneously separate and encode gold nano-triangles.By rational designing the DNA sequence,nano-triangles can be assembled on DNA origami and higher-order structures can be constructed.

关 键 词：纳米金颗粒 金三角片 DNA 自组装 电泳 纯化 

分 类 号：TB3[一般工业技术—材料科学与工程]