基于肠道微生态探讨理气化痰祛瘀方对高脂血症地鼠的调控作用  

Study on Regulating Effect of Liqi Huatan Quyu Decoction on Hyperlipidemia Hamsters Based on Intestinal Microecology

作　　者：丁科[1] 乐加薇 方明笋[3] 周小杰[3] 王辉[3] DING Ke;LE Jiawei;FANG Mingsun;ZHOU Xiaojie;WANG Hui(The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310005,China;First People’s Hospital of Yuhang District Hangzhou,Hangzhou 311100,China;Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江中医药大学附属第一医院,杭州310005 [2]杭州余杭区第一人民医院,杭州311100 [3]浙江中医药大学,杭州310053

出　　处：《中国现代应用药学》2019年第18期2249-2255,共7页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省中医药科技计划项目(2017ZB042)

摘　　要：目的观察理气化痰祛瘀方对高脂血症地鼠的干预作用及地鼠肠道菌群的变化。方法采用高脂饲料喂养诱导高脂血症金黄地鼠模型。将成模地鼠随机分为理气化痰祛瘀方低、中、高剂量组和非诺贝特组,检测各组地鼠总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-c)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-c)含量;HE染色观察肝脏病理变化;提取肠道微生物总DNA,检测肠道微生物菌群变化。结果理气化痰祛瘀方能显著降低TC、TG、LDL-c水平,改善肝脏病理变化程度。16S rDNA高通量测序技术结果显示,理气化痰祛瘀方干预后大鼠肠道菌群丰度及多样性均有一定程度改善,厚壁菌门和杆菌纲的相对丰度显著降低,而放线菌门、拟杆菌门显著升高。结论理气化痰祛瘀方能有效改善高脂血症金黄地鼠的血脂水平和肝脏病理变化,并在一定程度上恢复高脂血症金黄地鼠肠道菌群紊乱,其可能通过肠道菌群这一途径调节脂质代谢从而起到治疗高脂血症作用。OBJECTIVE To observe the intervention effect of Liqi Huatan Quyu decoction on hyperlipidemic hamsters,and explore the change of intestinal flora in hamsters.METHODS Hyperlipidemic golden hamster model was induced by high fat diet.The model hamsters were randomly divided into low,medium,high dose group and fenofibrate group.The contents of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-c),high density lipoprotein cholesterol(HDL-c)were measured;liver histopathologic changes were observed by HE staining;intestinal microorganism total DNA was extracted to detect intestinal microbial flora.RESULTS The Liqi Huatan Quyu decoction could reduce the serum TC,TG and LDL-c levels,improve the degree of liver pathological changes.The results of 16S rDNA high throughput sequencing showed that the abundance and diversity of intestinal microflora were improved to some extent.The relative abundance of phylum thuringiensis and bacteriaceae decreased significantly,while actinomycetes and bacteroides increased significantly.CONCLUSION Liqi Huatan Quyu decoction can effectively improve the lipid level and pathological changes of liver in hyperlipidemia golden hamster.And to some extent it can recover the intestinal flora disorder of hyperlipidemia golden hamster.It may play a role in the treatment of hyperlipidemia by regulating lipid metabolism through intestinal flora.

关 键 词：理气化痰祛瘀方 高脂血症 肠道菌群 

分 类 号：R285.5[医药卫生—中药学]