CO2超临界流体色谱法建立片姜黄指纹图谱检查方法的研究  

Study on the Fingerprint of Wenyujin Rhizoma Concisum by CO2-supercritical Fluid Chromatography

作　　者：唐登峰 黄琴伟 周颖 毛思浩 TANG Dengfeng;HUANG Qinwei;ZHOU Ying;MAO Sihao(Zhejiang Institute for Food and Drug Control,Hangzhou 310052,China)

机构地区：[1]浙江省食品药品检验研究院

出　　处：《中国现代应用药学》2019年第18期2285-2289,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省科技厅分析测试科技计划项目(2016C37064)

摘　　要：目的采用CO2超临界流体色谱法,建立片姜黄指纹图谱的检查方法。方法相似度测定采用ACQUITY UPC^2 HSS C18 SB色谱柱(3.0 mm×150 mm,1.8μm),以CO2-乙腈为流动相,梯度洗脱;流速为1.0 mL·min^-1;检测波长为216 nm,柱温为55℃,背压为2 000 psi。结果建立的片姜黄指纹图谱检查方法可用于片姜黄相似度的测定,15批样品的平均相似度为0.968,RSD为2.6%,而测定其他非正品来源的相似度均<0.400。结论建立的方法快捷、稳定且环境友好,能够较好地区分片姜黄和其他非正品。OBJECTIVE To develop the fingerprint of Wenyujin Rhizoma Concisum by CO2-supercritical fluid chromatography(CO2-SFC).METHODS The determination of similarity was performed on a ACQUITY UPC^2 HSS C18 SB analytical column(3.0 mm×150 mm,1.8μm)with CO2(A)-acetonitrile(B)as mobile phase in a gradient elution program at a flow rate of 1 mL·min^-1.The detection wavelength was 216 nm.The column temperature was maintained at 55℃.And the back pressure was 2 000 psi.RESULTS The established determination method for the fingerprint of Wenyujin Rhizoma Concisum could be used for the similarity determination on it,the average similarities of the 15 batches was 0.968 with RSD was 2.6%,while the unofficial samples were<0.400.CONCLUSION The developed method is simplify,rapidly,stability,and environmental friendly,which is helpful to identify the official and unofficial samples of Wenyujin Rhizoma Concisum.

关 键 词：CO2超临界流体色谱法 片姜黄 指纹图谱 

分 类 号：R917.101[医药卫生—药物分析学]