离子色谱法测定小儿复方氨基酸注射液中的氨含量  

Determination of Ammonium in Pediatric Compound Aminoacid Injection by Ion Chromatograpy

作　　者：傅萍 刘峰 谢华 FU Ping;LIU Feng;XIE Hua(Sichuan Food and Drug Inspection and Testing Institute,Chengdu 611731,China)

机构地区：[1]四川省食品药品检验检测院

出　　处：《中国现代应用药学》2019年第18期2295-2298,共4页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的采用离子色谱法优化测定小儿复方氨基酸注射液中的氨含量。方法采用阳离子交换色谱柱RFICTM IonPac^■CG16(4 mm×50 mm),保护柱CS16(4 mm×250 mm);以20 mmol·L^-1甲基磺酸为流动相A,以100 mmol·L^-1甲基磺酸为流动相B,梯度洗脱,流速为1.0 mL·min^-1;检测器为电导检测器(带抑制器CERS 300,4 mm);进样体积25μL。结果氨的线性范围为0.149 9~5.997 9μg·mL^-1(r=0.998 4),平均回收率为96.8%,RSD为2.82%。结论该法专属性高、结果准确可靠,可用于小儿复方氨基酸注射液中氨的含量控制。OBJECTIVE To use ion chromatography method to optimize the assay of ammonium in Pediatric Compound Aminoacid injection.METHODS The IC method was performed on a cation exchange column RFICTM IonPac^■CG16(4 mm×50 mm),CS16(4 mm×250 mm)by gradient elution at a flow rate of 1.0 mL·min^-1.The mobile phase A was 20 mmol·L^-1 methanesulfonic acid,the mobile phase B was 100 mmol·L^-1 methanesulfonic acid.The detector was conductivity detector with a suppressor(CERS 300,4 mm)and the injection volume was 25μL.RESULTS The linear range of the ammonium was 0.149 9-5.997 9μg·mL^-1(r=0.998 4).The average recovery of it was 96.8%and the RSD was 2.82%.CONCLUSION The method was specific,accurate and suitable for the assay of ammonium for quantitative control of Pediatric Compound Aminoacid injection.

关 键 词：离子色谱法 阳离子交换柱 电导检测器 小儿复方氨基酸注射液 氨 含量测定 

分 类 号：R917.101[医药卫生—药物分析学]