白香丹胶囊对PMDD肝气逆证模型大鼠血清抗焦虑样作用机制研究  

Anti-anxiety-like Mechanism of Baixiangdan Capsule on Serum of Rats with PMDD Liver-qi Syndrome

作　　者：王帅 郜彦鑫 张洪 付晓杰 刘金虎 张浩 王杰琼[1,2] 宋春红 Wang Shuai;Gao Yanxin;Zhang Hong(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Shandong 250355,China)

机构地区：[1]山东中医药大学药学院,济南250355 [2]山东中医药大学中医药经典理论教育部重点实验室,济南250355

出　　处：《医学研究杂志》2019年第9期66-70,共5页Journal of Medical Research

基　　金：国家自然科学基金青年科学基金资助项目(81403294、81603510);国家级大学生创新创业训练计划项目(201810441020);山东省中医药科技发展计划项目(2015-021)

摘　　要：目的以经前烦躁障碍症(premenstrual dysphoric disorder,PMDD)肝气逆证模型大鼠为载体探索白香丹胶囊对模型大鼠焦虑样行为的影响,并初步探究其血清作用机制。方法以居住入侵法制备PMDD肝气逆证模型大鼠并进行药物干预,给药后通过攻击行为测试、旷场实验、高架十字迷宫实验对PMDD肝气逆证模型大鼠焦虑样行为进行检测;腹主动脉取血并制备血清,使用ELISA法检测大鼠血清中孕酮(P)、四氢孕酮(ALLO)、γ-氨基丁酸(GABA)的含量变化。结果与正常组比较,模型组大鼠攻击行为明显增加(P<0.01);旷场实验,总路程增加而在中央区停留的总时间减少(P<0.01);高架试验,进入开放臂的次数百分比和时间百分比均明显减少(P<0.01);血清中P水平、ALLO含量、GABA水平均显著下降(P<0.05)。与模型组比较,氟西汀组和白香丹组大鼠的攻击行为均明显减小(P<0.01);旷场实验,总路程减少而在中央区停留的总时间均明显增加(P<0.01);高架试验,进入开放臂的次数百分比和时间百分比均明显增加(P<0.01);血清中P水平、ALLO水平、GABA水平均显著上升(P<0.05)。结论白香丹胶囊对PMDD肝气逆证模型大鼠有明显的抗焦虑样作用,并明显增加模型大鼠血清中的P、ALLO、GABA水平,据此推测该病证作用机制可能与调节体内P、ALLO、GABA的含量有关。Objective To investigated the effects of Baixiangdan Capsule on the anxiety-like behavior of model rats by using the model of pre-menstrual irritability and liver-qi syndrome.The mechanism of action was also explored.Methods The rats with PMDD liver-qi stagnation syndrome were prepared by residential invasion method and drug intervention was performed.After the administration,the anxiety behaviors of rats with PMDD liver-qi syndrome were tested by attack behavior test,open field test and elevated plus maze test for behavioral evaluation.The blood was taken from the posterior abdominal aorta and serum was prepared.The content of progesterone,tetrahydroprogesterone andγ-aminobutyric acid in the serum of rats was detected by ELISA method.Results Compared with the control,the attack behavior of the model group was significantly increased(P<0.01),the total distances increased and the central residence time decreased(P<0.01)in the open field text.The open arm entry and the open arm time of the elevated plus-maze test were significantly decreased(P<0.01).Serum P levels,ALLO levels and GABA levels were significantly decreased(P<0.05).Compared with the model group,the attack behaviors of the FXT group and the BXD group were significantly reduced(P<0.01),the total distances decreased and the central residence time increased significantly(P<0.01)in the open field text.Open arm entry and the open arm time of the elevated plus-maze test increased significantly(P<0.01).Serum P levels,ALLO levels and GABA levels increased significantly(P<0.05).Conclusion Baixiangdan Capsule has obvious anti-anxiety-like effect on PMDD liver-qi syndrome model rats,and significantly increases the levels of P,ALLO and GABA in the serum of model rats.It is speculated that the mechanism of action may be related to the regulation of P,ALLO and GABA in vivo.

关 键 词：白香丹胶囊 PMDD肝气逆证 焦虑 

分 类 号：R285.5[医药卫生—中药学]