小鼠子宫内膜表面蛋白的二维电泳研究  

Identify the Mouse Uterine Epithelial Surface Protein by Two-dimensional Electrophoresis

作　　者：叶天民 彭鼎佳 肖佳 杨树标 Ye Tianmin;Peng Dingjia;Xiao Jia(Center of Reproductive Medicine,The University of Hong Kong-Shenzhen Hospital,Guangdong 518053,China)

机构地区：[1]香港大学深圳医院生殖医学中心及生育调控重点实验室,518053 [2]暨南大学附属第一医院临床研究院,广州510632

出　　处：《医学研究杂志》2019年第9期90-94,共5页Journal of Medical Research

基　　金：广东省自然科学基金资助项目(2015A030310470)

摘　　要：目的运用生物素标记表面蛋白联合二维电泳技术发现小鼠子宫内膜腔上皮表面胚胎着床相关表面蛋白。方法对孕第1天(不接受胚胎阶段)和第4天(接受胚胎阶段)小鼠宫腔注入生物素进行标记,将抽提纯化后的表面蛋白进行二维电泳比较,发现的差异表达点用质谱进行分析确定,并用免疫组化方式进行验证。结果发现20个差异表达超过3倍的蛋白,其中接受胚胎阶段高表达蛋白13个,不接受胚胎阶段高表达蛋白7个。并用质谱发现确定了aminopeptidase N(APN)、annaxinA 2和intergrinβ1等蛋白。通过免疫组织化学方法验证了二维电泳的结果。结论笔者通过生物素标记小鼠子宫内膜腔上皮表面,并联合二维电泳发现了一些在接受胚胎着床阶段和不接受胚胎阶段差异表达的表面蛋白以及一些和胚胎着床相关的重要蛋白。Objective To identify mouse uterine epithelial surface proteins by biotin labeled with two-dimensional electrophoresis(2DE).Methods Biotin solution was infused into the day one(pre-receptive)and day 4(receptive)pregnant mice uterine cavity.The extracted and purified surface proteins from two group mice were compared by 2DE and identified by tandem mass spectrometry(MS/MS).The comparable protein expression was further confirmed by immunohistochemical study.Results A total of 20 proteins were identified,whose expression were three times than the other group.There were 13 proteins high-expressed in the receptive mouse and 7 proteins in the pre-receptive mouse.The proteins such as APN,annaxinA 2,intergrinβ1 etc.were identified by MS/MS.Immunohistochemical study was applied to confirm the protein expression pattern.Conclusion We had compared the proteome of biotin-labeled endometrial luminal epithelial surface between pre-receptive and receptive stage and identified a set of proteins specifically expressed in these stages.Some of them were demonstrated to be related to blastocyst attachment.

关 键 词：小鼠 表面蛋白 二维电泳 

分 类 号：R3[医药卫生—基础医学]