4株禽腺病毒hexon基因片段序列分析  

Sequence Analysis of hexon Gene Fragment of 4 Strains of Fowl Adenovirus

作　　者：刘畅[1] 李磊[1] 徐慧娟 赵磊[1] 路振香[1] 胡倩倩[1] LIU Chang;LI Lei;XU Hui-juan;ZHAO Lei;LU Zhen-xiang;HU Qian-qian(College of Animal Science,Anhui Science and Technology University,Fengyang 233100,China)

机构地区：[1]安徽科技学院动物科学学院

出　　处：《畜牧与饲料科学》2019年第9期17-22,共6页Animal Husbandry and Feed Science

基　　金：安徽省高校自然科学研究重点项目(KJ2017A504);安徽省高校自然科学研究重点项目(KJ2019A0801)

摘　　要：[目的]验证购自中国兽医微生物菌种保藏管理中心(China Veterinary Culture Collection Center,CVCC)的4株禽腺病毒(FAdV-1 AV208、FAdV-4 AV211、FAdV-8 AV215、FAdV-11 AV218)的血清型。[方法]采用PCR的方法对4株FAdV hexon基因片段进行特异性扩增、克隆与测序,并与GenBank上公布的FAdV各血清型进行亲缘性分析。[结果]hexon基因片段核苷酸及其编码Hexon蛋白氨基酸亲源性分析结果表明,FAdV-1 AV208为禽腺病毒A血清1型;FAdV-4 AV211为禽腺病毒C中的血清4型;FAdV-8 AV215为禽腺病毒E中的血清8b型;FAdV-11 AV218为禽腺病毒C中的血清10型。[结论]FAdV-1 AV208、FAdV-4 AV211的血清型与CVCC标注一致;FAdV-8 AV215为FAdV-8b,相比CVCC该研究进一步细化了其血清型;而FAdV-11 AV218为FAdV-10,与CVCC标注的血清型不一致。该研究为今后正确使用CVCC标准毒株FAdV-8 AV215、FAdV-11 AV218提供了参考。[Objective]To validate the serotype of 4 strains of fowl adenovirus(FAdV-1 AV208,FAdV-4 AV211,FAdV-8 AV215 and FAdV-11 AV218)purchased from China Veterinary Culture Collection Center(CVCC).[Method]The hexon gene fragments of the four strains of FAdV were specifically amplified by using PCR assay and were subsequently cloned and sequenced.The obtained nucleotide sequences and their corresponding amino acid sequences of the 4 strains were phylogenetically analyzed by comparing with those of FAdV strains of different serotypes published in GenBank.[Result]The phylogenetical analysis of nucleotide sequences of hexon gene fragments and their corresponding amino acid sequences demonstrated that the strains of FAdV-1 AV208,FAdV-4 AV211,FAdV-8 AV215 and FAdV-11 AV218 belonged to FAdV-A serotype-1,FAdV-C serotype-4,FAdV-E serotype-8b and FAdV-C serotype-10,respectively.[Conclusion]The serotypes of FAdV-1 AV208 and FAdV-4 AV211 were consistent with the label of CVCC;FAdV-8 AV215 was identified as FAdV-8b in this study,which further refined its serotype compared with the label of CVCC;FAdV-11 AV218 was determined as FAdV-10 in this study,which was inconsistent with the serotype labeled by CVCC.This study provided a reference for the correct use of CVCC standard strains of FAdV-8 AV215 and FAdV-11 AV218 in further study.

关 键 词：禽腺病毒 标准毒株 hexon基因 Hexon蛋白 

分 类 号：S852.659.1[农业科学—基础兽医学]