芪防膝痹颗粒对大鼠膝骨关节炎疼痛的影响  

Effects of Qifang Xibi Granules on pain of knee osteoarthritis in rats

作　　者：沈鹏 王腾腾[3] 张浩 沙南南 王文昊[1] 赵永见[3] 唐德志[3] 高翔[1] 保志军[1,4] 吴弢 SHEN Peng;WANG Teng-teng;ZHANG Hao;SHA Nan-nan;WANG Wen-hao;ZHAO Yong-jian;TANG De-zhi;GAO Xiang;BAO Zhi-jun;WU Tao(Huadong Hospital Affiiated to Fudan University,Shanghai 200040,China;Chongqing Traditional Chinese Medicine Hospital,Chongqing 400011,China;Institute of Spinal Disease,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Research Center on Aging and Medicine,Huadong Hospital Affiiated to Fudan University,Shanghai 200040,China)

机构地区：[1]复旦大学附属华东医院,上海200040 [2]重庆市中医院,重庆400011 [3]上海中医药大学脊柱病研究所,上海200032 [4]上海市老年医学临床重点实验室,上海200040

出　　处：《中华中医药杂志》2019年第9期3990-3994,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医药管理局“十二五”重点专科建设项目;上海市进一步加快中医药事业发展三年行动计划(No.ZY3-CCCX-3-3011);上海市中西医结合临床重大项目(No.ZY3-LCPT-2-2003)~~

摘　　要：目的:探讨芪防膝痹颗粒对大鼠膝骨关节炎(KOA)疼痛的影响。方法:选取16周龄SD雌性大鼠40只,分为对照组、模型组、塞来昔布和芪防膝痹颗粒组,每组10只。除对照组外其余行前交叉韧带切除建立KOA,术后分别给予0.9%氯化钠溶液、塞来昔布和芪防膝痹颗粒灌胃,8周后取材。Electric Von Frey疼痛仪于术前及术后每周测1次疼痛阈值,Micro-CT扫描后行HE、番红-快绿染色观察滑膜及软骨的改变,免疫组织化学观察IL-1β、TNF-α、VEGF的表达,ELISA测血清IL-6水平。结果:与模型组比较,芪防膝痹颗粒组KOA疼痛阈值显著升高(P<0.05),滑膜炎症和软骨破坏也明显减轻,VEGF在软骨边缘、滑膜以及IL-1β、TNF-α在软骨中表达均降低(P<0.05)。结论:芪防膝痹颗粒可以减轻KOA疼痛和延缓软骨退变,可能与减少滑膜中VEGF表达有关。Objective: To investigate the effect of Qifang Xibi Granules on pain of knee osteoarthritis(KOA) in rats. Methods: A total of 40 sixteen weeks of SD female rats were randomly divided into the control group, model group, celecoxib group and Qifang Xibi Granules group with 10 rats in every group. KOA was established by anterior cruciate ligament excision except the control group. Saline, celecoxib and Qifang Xibi Granules were given to the stomach after the operation. Samples were taken after 8 weeks. Electric Von Frey pain meter was used to measure pain before and after operation once a week. After micro-CT scanning, the changes of synovium and cartilage were observed by HE and SO. The expressions of IL-1β TNF-α and VEGF were observed by immunohistochemistry. Serum IL-6 levels were measured by ELISA. Results: Compared with the model group, the pain threshold of KOA in Qifang Xibi Granules group was significantly increased(P<0.05), synovitis and cartilage destruction in knee joints were significantly reduced, and the expression of VEGF in cartilage edge, synovium, IL-1β and TNF-α were all decreased(P<0.05). Conclusion: Qifangxibi Granule can relieve KOA pain and delay cartilage degeneration, which may be related to the decrease of the expression of vascular endothelial growth factor in synovium.

关 键 词：芪防膝痹颗粒 膝骨关节炎 疼痛 血管内皮生长因子 

分 类 号：R68[医药卫生—骨科学]