固本健脑法对AD大鼠模型海马Aβ1-42及SorLA、SNX27 mRNA表达的影响  

Effects of Guben Jiannao method on Aβ1-42 and expression of SorLA, SNX27 mRNA in hippocampus of AD rats

作　　者：李婧[1,2] 胡玉萍 袁德培[3] 曾楚华 LI Jing;HU Yu-ping;YUAN De-pei;ZENG Chu-hua(Hubei University of Chinese Medicine,Wuhan 430065,China;Wuchang District of Wuhan City Shuiguohu Street Community Health Service Centers,Wuhan 430061,China;School of Medicine,Hubei Institute for Nationalities,Enshi 445000,China)

机构地区：[1]湖北中医药大学,武汉430065 [2]武汉市武昌区水果湖街社区卫生服务中心,武汉430061 [3]湖北民族学院医学院,恩施445000

出　　处：《中华中医药杂志》2019年第9期4265-4268,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金青年科学基金项目(No.81503484)~~

摘　　要：目的:观察固本健脑法对阿尔茨海默病(AD)大鼠海马Aβ1-42含量及SorLA、SNX27 mRNA表达的影响。方法:84只12月龄Wistar大鼠随机分为正常组、空白对照组、模型组、固高组、固中组、固低组和西药组,除正常组、空白对照组外,各组行双侧海马注射Aβ1-42复制AD模型。经4周治疗,以Morris水迷宫检测学习记忆能力、ELISA法检测Aβ1-42含量,Real time-qPCR法检测SorLA、SNX27 mRNA表达。结果:与模型组比较,各用药组学习记忆能力显著提高(P<0.01,P<0.05),Aβ1-42表达显著降低(P<0.05,P<0.01),SorLA、SNX27 mRNA表达显著升高(P<0.05,P<0.01)。结论:固本健脑法可能通过上调模型大鼠海马SorLA、SNX27 mRNA水平,降低海马Aβ1-42含量,从而改善AD模型大鼠学习记忆能力。Objective: To observe the influence of Guben Jiannao method on content of Aβ1-42 and expression of SorLA, SNX27 mRNA in hippocampus of AD rats. Methods: A total of 84 rats which were 12 months old were were randomly divided into normal group, blank control group, model roup, Guben Jiannao Fluid high, middle, low dose group and western medicine group. The model group and treatment groups were given injection concentration of Aβ1-42 in hippocampal to establish AD model. After four weeks treatment, the learning and memory ability was tested by Morris water maze test in rats. The level of Aβ1-42 in hippocampus was detected by ELISA. The expression of SorLA and SNX27 in hippocampus were detected by quantitative Real time q-PCR. Results: Compared with model group, the learning and memory abilities of treatment groups increased significantly(P<0.01, P<0.05), Aβ1-42 expression decreased(P<0.05, P<0.01), and SorLA, SNX27 mRNA expression increased(P<0.05, P<0.01) in treatment groups. Conclusion: The Guben Jiannao method may decrease the contents of Aβ1-42 and improve expressions of SorLA, SNX27 mRNA in hippocampus, so as to ameliorate the learning and memory ability of AD model rats.

关 键 词：固本健脑法 阿尔茨海默病 Β淀粉样蛋白 SorLA SNX27 

分 类 号：R28[医药卫生—中药学]