白背叶根水提物对大鼠肝星状细胞活化及细胞外基质分泌的影响  

Effects of water extract of Mallotus apelta(Lour.) Muell.-Arg. root on hpatic stellate cells activation and ECM secretion in rats

作　　者：章波 檀燕君 黄秋洁 王捷[1] 叶勇[1] 梁秋云[1] ZHANG Bo;TAN Yan-jun;HUANG Qiu-jie;WANG Jie;YE Yong;LIANG Qiu-yun(School of Pharmacy,Guangxi Medical University,Nanning 530021,China;School of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530001,China)

机构地区：[1]广西医科大学药学院,南宁530021 [2]广西中医药大学药学院,南宁530001

出　　处：《中华中医药杂志》2019年第9期4287-4290,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81360689);广西自然科学基金项目(No.2015GXNSFAA139173);南宁市科技开发计划重点项目(No.20173158-4);广西中医药大学“杨世林教授团队人才培养”建设项目(No.YSL17009)~~

摘　　要：目的:探讨白背叶根(MMAR)水提物对四氯化碳(CCl4)诱导大鼠肝纤维化的保护机制。方法:将大鼠随机平均分为6组:对照组,模型组,秋水仙碱组(0.2mg/kg),MMAR高、中、低剂量组(分别相当于生药5、2.5、1.25g/kg),每组12只,对照组和模型组给予等量0.9%氯化钠溶液。实验结束后,采集血液和肝脏。使用全自动生化分析仪检测大鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)水平;试剂盒检测血清中超氧化物歧化酶(SOD)、丙二醛(MDA)、羟脯氨酸(Hyp)水平;HE和Masson染色观察肝脏病理学改变;q-PCR测定Col-Ⅰ、TIMP-1、TGF-β1、α-SMA mRNA表达。结果:与模型组比较,MMAR各剂量组血清中ALT、AST、MDA和Hyp的水平明显降低(P<0.05),SOD活性明显升高(P<0.05),NMAR高、中剂量组Col-Ⅰ、TIMP-1、TGF-β1、α-SMA mRNA表达被显著抑制(P<0.05)。结论:MMAR可以通过抑制氧化应激、恢复MMPs和TIMPs之间的平衡、抑制HSC活化等途径显著减轻CCl4诱导的肝纤维化。Objective: To investigate the protective effect of water extract of Mallotus apeltan(Lour.) Muell.-Arg. root(MMAR) on CCl4-induced hepatic fibrosis, exploring the underlying mechanism of MMAR against liver fibrosis. Methods: Rats were randomly divided into six groups(12 in each group): normal control group, model group, colchicine group(0.2 mg/kg), MMAR high-, medium-, low-dose treatment group(5, 2.5, 1.25 g/kg). The rats in normal control group and model group were given equivalent 0.9% sodium chloride solution. At the end of the experiment, the blood and liver samples were collected for the further examination. The levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in serum were detected by using an automatic biochemical analyzer. The levels of superoxide dismutase(SOD), malondialdehyde(MDA), and hydroxyproline(Hyp) in serum were detected by commercial assay kits. The hepatic histopathological changes were observed by HE and Masson staining. Additionally, the mRNA expression of Col-Ⅰ, TIMP-1, TGF-β1 and α-SMA were measured by real-time quantitative PCR. Results: Compared with the model group, treatment with MMAR could significantly decrease the levels of ALT, AST, MDA and Hyp(P<0.05), meanwhile SOD was significantly increased(P<0.05). The mRNA expressions of liver fibrosis biomarkers including Col-Ⅰ, TIMP-1, TGF-β1 and α-SMA in high, medium-dose treatment group were significantly decreased(P<0.05). Conclusion: MMAR significantly alleviates CCl4-induced hepatic fibrosis by inhibiting oxidative stress, restoring the balance between MMPs and TIMPs, and suppressing HSC activation.

关 键 词：白背叶根水提取物 肝纤维化 氧自由基 肝星状细胞 ECM 

分 类 号：R28[医药卫生—中药学]