右美托咪定对小鼠皮肤/肌肉切口牵拉所致持续性术后疼痛的作用  

Effect of dexmedetomidine on persistent postoperative pain induced by skin/muscle incision and retraction in mice

作　　者：杨娟 周璟 郭小文[1] YANG Juan;ZHOU Jing;GUO Xiaowen(Department of Anesthesiology,Zhejiang Provincial Hospital of TCM,Hangzhou 310006,China)

机构地区：[1]浙江省中医院麻醉科

出　　处：《浙江医学》2019年第19期2045-2048,共4页Zhejiang Medical Journal

摘　　要：目的通过疼痛行为学检测评估小鼠皮肤/肌肉切口牵拉模型是否造模成功并观察右美托咪定对该模型小鼠疼痛行为学的影响。方法(1)将实验鼠随机分为皮肤/肌肉切口牵拉组(SMIR组)和皮肤/肌肉只切开不牵拉组(Sham组),在术前1d、术后第1、3、7、10、14、18天检测其机械缩足反射阈值及热缩足潜伏期,评估该模型是否造模成功。(2)观察右美托咪定对SMIR小鼠疼痛行为学的影响:于术后第3天腹腔注射不同剂量右美托咪定(0.4、2、10μg/kg)及0.9%氯化钠注射液,观察其对SMIR小鼠触诱发痛的影响;于术后第4天腹腔注射10μg/kg右美托咪定,观察是否引起SMIR小鼠条件性位置偏爱以检测右美托咪定对SMIR小鼠自发痛的影响。结果(1)与Sham组相比,SMIR组造模前后机械痛阈值从术后1d开始明显下降,至少持续到术后第14天,术后第18天恢复正常;热痛潜伏期无明显变化。(2)与0.9%氯化钠注射液相比,右美托咪定可以提高SMIR小鼠机械痛阈值并呈剂量依赖性;且10μg/kg右美托咪定能改善SMIR小鼠的自发痛。结论小鼠SMIR建立持续性术后疼痛模型简单易行且具有一定的稳定性;右美托咪定可以改善SMIR小鼠的触诱发痛及自发痛。Objective To investigate the effect of dexmedetomidine on persistent postoperative pain induced by skin/muscle incision and retraction(SMIR) in mice. Methods Mice were randomly assigned to 2 groups: skin/muscle incision and retraction(SMIR) group and skin/muscle incision without retraction(Sham) group. Paw withdrawal thresholds(PWT) to mechanical stimuli and heat stimuli were recorded at d1 before surgery, d1, d3, d7, d10, d14 and d18 after surgery.Dexmedetomidine(0.4, 2, 10μg/kg) was injected on d3 after surgery, the pain was assessed by mechanical PWT. The effect of dexmedetomidine on the spontaneous pain was evaluated by using a conditional place preference(CPP) behavioral paradigm. The mice were systemically injected with dexmedetomidine(10μg/kg) on d4 after surgery, then CPP was observed.Results SMIR-evoked mechanical hypersensitivity was observed at postoperative d1, persisted until at least postoperative d14, and had dissipated by postoperative d18. SMIR did not evoke significant heat hyperalgesia. Systemic injection of dexmedetomidine raised the PWT in SMIR mice in a dose-dependent manner. Systemic injection of 10μg/kg dexmedetomidine alleviated spontaneous pain in the CPP test. Conclusion The study suggests that skin/muscle incision and retraction model in mice evokes a persistent pain syndrome. Dexmedetomidine can relieve the evoked pain and spontaneous pain in mice.

关 键 词：持续性术后疼痛 自发痛 条件性位置偏爱 右美托咪定 

分 类 号：R28[医药卫生—中药学]