草地贪夜蛾莱氏绿僵菌的分离鉴定  

Isolation and identification of entomopathogenic fungi Metarhizium rileyi isolated from Spodoptera frugiperda (J.E.Smith)

作　　者：郑亚强[1] 胡惠芬 付玉飞 金新华 张栩 杨宝云 张志红 黄明亮 李永川[2] 陈斌[1] 李正跃[1] ZHENG Yaqiang;HU Huifen;FU Yufei;JIN Xinhua;ZHANG Xu;YANG Baoyun;ZHANG Zhihong;HUANG Mingliang;LI Yongchuan;CHEN Bin;LI Zhengyue(College of Plant Protection,Yunnan Agricultural University,State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Kunming 650201,China;Yunnan Plant Protection and Plant Quarantine Station,Kunming 650034,China)

机构地区：[1]云南农业大学植物保护学院,云南生物资源保护与利用国家重点实验室,昆明650201 [2]云南省植保植检站,昆明650034

出　　处：《植物保护》2019年第5期65-70,共6页Plant Protection

基　　金：国家重点研发计划(2018YFD0200703);云南省大学生创新训练计划项目(S201910676043)

摘　　要：草地贪夜蛾Spodoptera frugiperda(J.E.Smith)是2019年入侵我国的一种迁飞性害虫,已成为严重影响我国玉米生产的重要害虫。目前国内关于草地贪夜蛾昆虫病原真菌的研究报道很少。2019年6月,在云南省曲靖市沾益县安东村玉米田调查发现了被虫生真菌感染的草地贪夜蛾幼虫。经室内分离培养,结合形态和ITS序列相似性分析鉴定确认,感染草地贪夜蛾幼虫病原性真菌为莱氏绿僵菌Metarhizium rileyi,编号为ZYSP190701菌株。调查表明,2019年7月17日田间草地贪夜蛾的莱氏绿僵菌感染率为15.46%±2.52%,8月2日感染率达29.83%±13.71%。用莱氏绿僵菌ZYSP190701菌株1×10^8个/mL孢子浓度接种草地贪夜蛾3龄幼虫后第7天时草地贪夜蛾的感染死亡率高达100%。上述结果表明,莱氏绿僵菌ZYSP190701对草地贪夜蛾有良好的生防潜力,具有草地贪夜蛾生防菌剂开发应用的前景。Spodoptera frugiperda(J.E.Smith)is an outbreak invasive pest,especially for maize.To date,little information about entomopathogenic fungi of Spodoptera frugiperda is available in China.In this study,fungi infected S.frugiperda larvae were found in Andong village,Zhanyi county,Qujing city,Yunnan province.The fungi were isolated from the infected cadaver of S.frugiperda,and then the species was identified based on the morphological and molecular biology,the virulence of the fungi against the larvae of S.frugiperda was tested.Results showed that this strain was identified as Metarhizium rileyi(No.ZYSP190701).The infection rate of S.frugiperda was 15.46%±2.52%on 17th,July and 29.83%±13.71%on 2nd,August,respectively.The cumulative mortality of the 3rd instar larvae of S.frugiperda 7 d after immersion inoculation at 1×10^8 conidia/mL was 100%.Our results demonstrated that M.rileyi ZYSP190701 is a promising candidate for S.frugiperda biocontrol in field.

关 键 词：草地贪夜蛾 莱氏绿僵菌 昆虫病原真菌 生防菌剂 

分 类 号：S435.132[农业科学—农业昆虫与害虫防治]