大白菜软腐病拮抗菌的筛选、鉴定及其防效研究  

Screening, identification and control effect of the antibacteria against soft rot of Chinese cabbage

作　　者：王彪[1] 崔文艳[1] 武君洁 何朋杰 吴毅歆[1,2] 何鹏飞 王再强[1] 李兴玉 何月秋[1,2] WANG Biao;CUI Wenyan;WU Junjie;HE Pengjie;WU Yixin;HE Pengfei;WANG Zaiqiang;LI Xingyu;HE Yueqiu(Yunnan Agricultural University,Kunming 650201,China;National and Local Joint EngineeringResearch Center for Screening and Application of Microbial Strains,Kunming 650217,China)

机构地区：[1]云南农业大学,昆明650201 [2]微生物菌种筛选与应用国家地方联合工程研究中心,昆明650217

出　　处：《植物保护》2019年第5期157-162,共6页Plant Protection

基　　金：科技部国际合作计划项目(2009DFA32360)

摘　　要：为筛选对大白菜软腐病具有较好拮抗效果的生防菌株,采用对峙平板法分别从作物根际土、发酵池以及本实验室菌种库得到6株拮抗性较强的菌株ASR-12、ASR-23、ASR-31、ASR-54、ASR-55、ASR-150。在离体叶柄软腐防效试验中,采用注射接种法获得了拮抗性较强的3株拮抗菌ASR-12、ASR-23和ASR-150,48 h的防效分别达到了87.64%、80.90%和70.79%。温室防效试验的结果表明:菌株ASR-12、ASR-23和ASR-150防治效果分别为59.22%、59.21%和55.00%,显著高于72%农用链霉素可湿性粉剂的防效(39.15%),具有很好应用前景。依据形态、生理生化和分子鉴定,菌株ASR-12被鉴定为解淀粉芽胞杆菌Bacillus amyloliquefaciens。In order to screen the antagonistic strains with good effects on soft rot of Chinese cabbage,a total of 6 strong antagonistic strains,ASR-12,ASR-23,ASR-31,ASR-54,ASR-55 and ASR-150,were isolated from the rhizosphere soil of the crops in the greenhouse,fermentation pool and bacterial bank through confrontation plate culture.Three antagonistic strains,ASR-12,ASR-23 and ASR-150,were selected for soft rot resistance test by petiole injection method of Chinese cabbage in vitro,and their control effects were 87.64%,80.90%and 70.79%,respectively,in 48 h.The three strains were tested against soft rot in the greenhouse by spraying method,and the results showed that the control effects of the strains ASR-12,ASR-23 and ASR-150 were 59.22%,59.21%and 55.00%,respectively,significantly better than 72%agricultural streptomycin wettable powders whose effect was 39.15%,indicating they have good application prospect.Based on morphological,physiological and biochemical and molecular identification,the strain ASR-12 was identified as Bacillus amyloliquefaciens.

关 键 词：拮抗菌 筛选 防效 鉴定 解淀粉芽胞杆菌 

分 类 号：S436.341.13[农业科学—农业昆虫与害虫防治]