九叶青花椒根结线虫病的病原鉴定  

Identification of root-knot nematode on Zanthoxylum armatum var. novemfolius

作　　者：曹业凡 汪来发[1] 王曦茁[1] 徐志伦 李央 姚玲 CAO Yefan;WANG Laifa;WANG Xizhuo;XU Zhilun;LI Yang;YAO Ling(Key Laboratory of Forest Protection of State Forestry Administration,Research Institute of Forest Ecology,Environment and Protection,CAF,Beijing 100091,China;Forestry Bureau of Pengxi County,Sichuan Province,Pengxi 629100,China;Forestry Bureau of Suining,Sichuan Province,Suining 629000,China)

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点实验室,北京100091 [2]四川省蓬溪县林业局,蓬溪629100 [3]四川省遂宁市林业局,遂宁629000

出　　处：《植物保护》2019年第5期242-246,共5页Plant Protection

基　　金：林业科学技术推广项目([2016]09);国家微生物资源平台(NIMR-2018-7)

摘　　要：为确定四川省蓬溪县九叶青花椒种植区发病植株花椒根结线虫的种类,进而为九叶青花椒根结线虫病防治提供依据,本文根据根结线虫雌虫与2龄幼虫的形态特征及雌成虫的会阴花纹、雌虫酯酶同工酶图谱,并结合特异性扩增及rDNA-ITS扩增,根据ITS序列构建系统发育树,对该地区九叶青花椒根结线虫病的病原进行了种类鉴定。结果表明该病原为南方根结线虫Meloidogyne incognita(Kofold&White)Chitwood。这是我国首次在九叶青花椒上发现南方根结线虫。In order to confirm the species of root-knot nematode on Zanthoxylum armatum var.novemfolius in Pengxi County,Sichuan province and provide the reference for prevention and controlling with root-knot disease,the nematode was identified by morphological observation,biochemical observation,specific amplification and rDNA-ITS sequence analysis.The results showed that the root-knot nematode on Z.armatum var.novemfolius was Meloidogyne incognita(Kofold&White)Chitwood.This is the first report of M.incognita on Z.armatum var.novemfolius in China.

关 键 词：九叶青花椒 南方根结线虫 RDNA-ITS 

分 类 号：S435.73[农业科学—农业昆虫与害虫防治] S432.45[农业科学—植物保护]