光谱对红鳍东方鲀仔稚鱼生长及相关基因表达量的影响  

Effects of light spectrum on growth and related gene expression of larval and juvenile tiger puffer Takifugu rubripes

作　　者：魏平平 李鑫 费凡[1,2] 马贺 高东奎 宋昌斌 陈涛[4] 刘鹰 of larval and juvenile tiger puffer Takifugu rubripesWEI Ping-ping;LI Xin;FEI Fan;MA He;GAO Dong-kui;SONG Chang-bin;CHEN Tao;LIU Ying(College of Marine Science and Environment,DaLian Ocean University,Dalian 116023,China;Key Laboratory of Environment Controlled Aquaculture Ministry of Education,Dalian 116023,China;Institute of Semiconductors China Academy of Sciences,Beijing 100083,China;Led Cooler Technology Company Limited,Shenzhen 518000,China;Qingdao National Laboratory for Marine Science and Technology,Qingdao 266000,China)

机构地区：[1]大连海洋大学海洋科技与环境学院,辽宁大连116023 [2]设施渔业教育部重点实验室,辽宁大连116023 [3]中国科学院半导体研究所,北京100083 [4]深圳市超频三科技股份有限公司,广东深圳518000 [5]青岛海洋科学与技术国家实验室,山东青岛266000

出　　处：《大连海洋大学学报》2019年第5期668-673,共6页Journal of Dalian Ocean University

基　　金：国家重点研发计划项目(2017YFB0404000)

摘　　要：为了解光谱对红鳍东方鲀Takifugu rubripes仔稚鱼(200尾,体质量为1.86 mg±0.48 mg)生长及相关基因表达量的影响,采用实时荧光定量PCR技术,对不同光谱处理后第12、22天仔稚鱼的生长激素基因GH(Growth hormone)、生长激素受体1型基因GHR1(Growth hormone receptor 1)、类胰岛素生长因子1型基因IGF1(Insulin-like growth factor 1)、生长激素释放激素基因1型GHRH1(Growth hormone-relasing hormone 1)和生长激素抑制激素1型基因SS1(Somatostatin 1)相对表达量变化情况进行了研究。结果表明:经不同光谱处理后,黄光组(λ590~595 nm)仔稚鱼的体长、体质量比其他光色组增加较多,其次是蓝光(λ450~455 nm)、白光(λ400~780 nm)、绿光(λ525~530 nm)组,不同光色对仔稚鱼生长有显著性影响(P<0.05);12 d时,红光组(λ625~630 nm)的GH基因相对表达量显著高于蓝光、黄光、白光组(P<0.05),绿光组GHR1基因相对表达量显著高于其他光色组(P<0.05),绿光组IGF1基因相对表达量显著高于白光组(P<0.05),绿光和红光组GHRH1基因相对表达量显著高于白光、黄光组(P<0.05),红光组SS1基因相对表达量显著高于其他光色组(P<0.05),SS1基因表达量由大到小依次为红光组>蓝光组>绿光组>白光组>黄光组;22 d时,红光组仔稚鱼几乎全部死亡,绿光组GH基因相对表达量著高于蓝光、黄光组(P<0.05),不同光色处理组的GHR1、GHRH1基因相对表达量均无显著性差异(P>0.05),绿光组IGF1基因相对表达量显著高于其他光色组(P<0.05),黄光组SS1基因相对表达量显著高于其他光色组(P<0.05)。研究表明,黄光对红鳍东方鲀仔稚鱼生长、发育具有促进作用,红光、绿光不适合红鳍东方鲀仔稚鱼生存。Postlarval tiger puffer Takifugu rubripes with body length of(4.09±0.39)mm were reared in a round barrel with 80 cm in diameter and 50 cm in height under various light spectra from LED emission including white(λ400-780 nm),blue(λ450-455 nm),green(λ525-530 nm),yellow(λ590-595 nm),and red(λ625-630 nm)to explore the effects of light spectrum on growth and related gene expression of tiger puffer larvae.The changes in relative expression levels of the related genes including growth hormone gene,growth hormone receptor 1 gene,insulin-like growth factor 1 gene,growth hormone-relasing hormone 1 gene and somatostatin 1 gene were detected by real-time fluorescence quantitative PCR.The results showed that there were higher body length and body weight in the larval tiger puffer exposed to yellow light spectrum than those in other light colors,followed by the blue,white and green light exposure fishes,with significant differences among different light colors(P<0.05).The expression level of GH gene was found to be significantly higher in red light group than that in blue,yellow and white groups at 12 d(P<0.05),without significant difference between the two groups(P>0.05).The expression level of GHR1 gene was significantly higher in green light group than that in other groups at 12 d(P<0.05).The expression levels of IGF1 gene were shown to be significantly higher under green light,yellow light and red light than those under green light and white light(P<0.05),and the expression levels of GHRH1 gene were significantly higher in green light and red light groups than those in white and yellow light groups at 12 d(P<0.05).The expression level of SS1 gene was significantly higher in red light group than that in other treatment groups(P<0.05),with the descending order as red>blue>green>white>yellow.At 22 d,however,almost all of the larvae were found to be died in the red light group,while the expression level of GH gene was higher in the green light group than that in the blue and yellow light groups(P<0.05),without significant

关 键 词：红鳍东方鲀 光谱 生长相关基因(GH、GHR1、IGF1、GHRH1、SS1) 

分 类 号：S965.335[农业科学—水产养殖]