添加槐花、香蕉花和杭白菊对体外诱导人工瘤胃酸中毒缓解效果的研究  

Alleviating Effects of Adding Sophora japonica,Musa nana flower and Chrysanthemum morifolium on Induced Artificial Rumen Acidosis in Vitro

作　　者：范曜天 孙劼[1] 夏光亮 赵芳芳 孙华 王洪荣[1] FAN Yaotian;SUN Jie;XIA Guangliang;ZHAO Fangfang;SUN Hua;WANG Hongrong(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学动物科学与技术学院

出　　处：《动物营养学报》2019年第10期4757-4765,共9页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：江苏省现代农业(肉羊)产业技术体系(JATS[2018]305);国家自然科学基金项目(31872988,31572429);扬州大学高端人才支持计划(2016)

摘　　要：本试验旨在研究添加槐花、香蕉花和杭白菊对体外诱导人工瘤胃酸中毒的缓解效果,为实际生产中缓解瘤胃酸中毒的应用提供理论依据。试验采用体外批次培养法,分别在高非纤维性碳水化合物底物[非纤维性碳水化合物/中性洗涤纤维(NFC/NDF)为2.30]和常规底物(NFC/NDF为1.19)中添加槐花、香蕉花和杭白菊,研究其对于瘤胃发酵参数的影响。试验采用单因素设计,试验分4组,每组设置3个重复。3种植物的添加量均为10 mg/mL,对照组不添加植物干粉,分别于发酵后的3、6、9、12 h,采集发酵液用于各发酵参数的测定。结果表明:1)常规底物中,香蕉花组各发酵阶段的体外发酵液pH均高于对照组(P>0.05);高非纤维性碳水化合物底物中,对照组各发酵阶段的体外发酵液pH显著高于杭白菊组(P<0.05)。2)常规底物中,发酵3、6 h时,香蕉花组的体外发酵液乳酸浓度显著高于对照组(P<0.05);发酵12 h时,槐花组、香蕉花组和杭白菊组的体外发酵液乳酸浓度均显著低于对照组(P<0.05)。高非纤维性碳水化合物底物中,发酵3、6 h时,香蕉花组的体外发酵液乳酸浓度显著高于对照组(P<0.05);发酵12 h时,杭白菊组的体外发酵液乳酸浓度显著低于对照组(P<0.05)。3)发酵12 h时,各组的体外发酵液氨态氮浓度无显著差异(P>0.05),但发酵过程中存在差异。4)常规底物中,发酵12 h时,槐花组和杭白菊组的体外发酵液总挥发性脂肪酸浓度和丙酸比例显著高于对照组和香蕉花组(P<0.05),乙酸和丁酸比例显著低于对照组和香蕉花组(P<0.05)。高非纤维性碳水化合物底物中,发酵12 h时,杭白菊组的体外发酵液总挥发性脂肪酸浓度显著高于对照组和香蕉花组(P<0.05),乙酸、丙酸、丁酸比例与对照组无显著差异(P>0.05)。由此可见,杭白菊能缓解瘤胃乳酸累积,而香蕉花则具有调控瘤胃pH相对衡定的能力,因此香蕉花和杭白菊在缓解瘤胃酸中毒�The aim of this experiment was to investigate the alleviating effect of adding Sophora japonica,Musa nana flower and Chrysanthemum morifolium on induced artificial rumen acidosis in vitro,and to provide a theoretical basis for the application of alleviating rumen acidosis in production.The experiment used in vitro batch culture to study the effects of adding Sophora japonica,Musa nana flower and Chrysanthemum morifolium on rumen fermentation parameters in substance with high non-fibrous carbohydrates substance[non-fibrous carbohydrates/neutral detergent fiber(NFC/NDF)was 2.30]and conventional substance(NFC/NDF was 1.19),respectively.Single factor design was used in the experiment,the experiment was divided into 4 groups,and each group was repeated 3 times.Three plants were added 10 mg/mL in rumen fluid,and the control group did not add plant powder.Samples were collected on 3,6,9 and 12 h cultured in vitro and fermentation parameters were measured.The results showed as follows:1)in conventional substance,the pH at each fermentation stage of Musa nana flower group was higher than that of the control group(P>0.05);in high non-fibrous carbohydrates substance,the pH at each fermentation stage of control group was significantly higher than that of Chrysanthemum morifolium group(P<0.05).2)In conventional substance,at fermentation 3 and 6 h,the concentration of lactic acid in fermentation fluid in vitro of Musa nana flower group was significantly higher than that of the control group(P<0.05);at fermentation 12 h,the concentration of lactic acid in fermentation fluid in vitro of Sophora japonica group,Musa nana flower group and Chrysanthemum morifolium group was significantly lower than that of the control group(P<0.05).In high non-fibrous carbohydrates substance,at fermentation 3 and 6 h,the concentration of lactic acid in fermentation fluid in vitro of Musa nana flower group was significantly higher than that of the control group(P<0.05);at fermentation 12 h,the concentration of lactic acid in fermentation fluid in vit

关 键 词：槐花 香蕉花 杭白菊 瘤胃酸中毒 

分 类 号：S816.5[农业科学—饲料科学]