新月弯孢霉CICC40301催化4–烯–3–酮甾体化合物羟基化反应产物的鉴定  

Identification of Hydroxylation Products of 4-alkene-3-one Steroids Catalyzed by Curvularia lunata CICC40301

作　　者：李慧杰 李明[1] 金鹏 郭文丽 王雪 王正祥 刘晓光 LI Huijie;LI Ming;JIN Peng;GUO Wenli;WANG Xue;WANG Zhengxiang;LIU Xiaoguang(College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;College of Chemical Engineering and Materials Science,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津科技大学化工与材料学院,天津300457

出　　处：《天津科技大学学报》2019年第5期35-38,44,共5页Journal of Tianjin University of Science & Technology

基　　金：天津市高等学校创新团队培养计划资助项目(TD12–5002)

摘　　要：丝状真菌是工业上甾体微生物羟基化反应菌种的主要来源.本文首次报道新月弯孢霉(Curvularia lunata)转化孕酮、雄甾-4-烯-3,17-二酮和睾酮及其对应产物.利用高效液相色谱(HPLC)和核磁共振方法分析了相应的主要转化产物,鉴定结果为:C17α-羟基孕酮、C14α-羟基4AD、C15-羟基睾酮,其中新月弯孢霉CICC40301转化孕酮生成的产物C17α-羟基孕酮是用于合成氢化可的松等多种甾体药物的重要中间体,C14α-羟基4AD是合成抗肿瘤化合物C14α-羟基-3,6,17三酮的关键前体.Filamentous fungi are the main source of industrial strains for steroid hydroxylation reaction.This paper reports for the first time on the transformation of progesterone,androst-4-ene-3,17-dione and testosterone catalyzed by Curvularia lunata and their major transformation products.The transformation products,characterized with high performance liquid chromatography(HPLC)and nuclear magnetic resonance(NMR)method,were identified to be C17α-hydroxyprogesterone,C14α-hydroxy-4AD and C15-hydroxytestosterone.17α-OH-progesterone is an important intermediate for the synthesis of hydrocortisone and other steroidal drugs;14α-hydroxy-4-androstene-3,17-dione is a key precursor for the production of 14α-hydroxy-4-androstene-3,6,17-trione,an antitumor agent.

关 键 词：新月弯孢霉 孕酮 睾酮 雄甾-4-烯-3 17-二酮 

分 类 号：Q781[生物学—分子生物学]