萱草叶枯病菌粗毒素液对分化芽生理生化代谢的影响  

Effect of Toxin Liquid of Kabatiella microsticta Extracted from Hemerocailis hybridai on Physiology,Biochemistry and Metabolism of Differentiated Buds

作　　者：杨丽莉[1] 杨睿[1] 常建忠[1] 蒋丹 YANG Lili;YANG Rui;CHANG Jianzhong;JIANG Dan(Research Center of Dryland Farming,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)

机构地区：[1]山西省农业科学院旱地农业研究中心

出　　处：《山西农业科学》2019年第10期1725-1729,共5页Journal of Shanxi Agricultural Sciences

基　　金：山西省重点研发计划项目(201603D221017-1);山西省农业科学院特色农业攻关项目(YGG17037)

摘　　要：研究了萱草叶枯病菌毒素液对大花萱草愈伤组织分化芽不同生长时间内生长量、成活率和保护酶(SOD、POD、CAT)活性的影响。结果表明,随着病菌毒素液剂量的增加毒害作用增强;添加40%叶枯病菌毒素液为抗病筛选的半致死剂量,培养24 d后,成活率达50%左右;叶枯病菌毒素液对SOD、POD和CAT这3种酶活性的影响均表现为先升高后降低,活性升高的程度与病菌毒素液的浓度呈正相关,其中,POD酶活性峰值出现在第7天,SOD和CAT酶活性峰值出现在第2天。In this paper,the impact of bacterial toxin liquid of Kabatiella microsticta extracted from Hemerocailis hybridai on the amount of growth,survival rate and protective enzymes(SOD,POD,CAT)activity of Hemerocailis hybridai callus differentiation buds was studied at different growth times.The result showed that the toxic liquid effect enhanced with the increase of toxin dose.40%of Kabatiella microsticta toxin liquid was added to be half lethal dose for disease resistance screening.24 d after culture,the survival rate reached approximately 50%.The impact of Kabatiella microsticta toxin liquid on the enzymatic activity of SOD,POD and CAT showed increase first and then decrease in different levels.The degree of increased activity was positively correlated with concentration of Kabatiella microsticta toxin liquid.The peak value of POD enzymatic activity was found in the 7th day,while SOD and CAT appeared on the 2nd day.

关 键 词：大花萱草 萱草叶枯病菌 病菌毒素液 生长状态 保护酶活性 

分 类 号：S682.19[农业科学—观赏园艺]