长尾仓鼠SSR位点的筛选与扩增  

Screening and Amplification of SSR Site in Cricetulus longicaudatus

作　　者：杨新根 张育平[5] 郭红芳[1,2] 张建珍 武振荣[6] 王庭林 邹波 常文英[3,4] 侯玉 YANG Xin'gen;ZHANG Yuping;GUO Hongfang;ZHANG Jianzhen;WU Zhenrong;WANG Tinglin;ZOU Bo;CHANG Wenying;HOU Yu(Institute of Applied Biology,Shanxi University,Taiyuan 030006,China;College of Life Science,Shanxi University,Taiyuan 030006,China;Institute of Plant Protection,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China;Shanxi Key Laboratory of Integrated Pest Management in Agriculture,Taiyuan 030031,China;Department of Biology,Taiyuan Normal University,Taiyuan 030031,China;Forestry Workstation of Wutai County,Wutai 035500,China)

机构地区：[1]山西大学应用生物学研究所,山西太原030006 [2]山西大学生命科学学院,山西太原030006 [3]山西省农业科学院植物保护研究所,山西太原030031 [4]农业有害生物综合治理山西省重点实验室,山西太原030031 [5]太原师范学院生物系,山西太原030031 [6]五台县林业工作站,山西五台035500

出　　处：《山西农业科学》2019年第10期1762-1765,共4页Journal of Shanxi Agricultural Sciences

基　　金：山西省重点研发计划项目(201803D221015-1);山西省农业科学院特色农业技术攻关项目(YGG17110);山西省农业科技成果转化和示范推广项目(2017CGZH47)

摘　　要：利用从山西省11个地市共计13个县(市)野外捕捉的132只长尾仓鼠为试验材料,先提取其基因组DNA,然后应用查阅的近源种黑线仓鼠的6对SSR位点引物(已登记在GeneBank)对长尾仓鼠基因组DNA进行PCR扩增,并对扩增产物进行8%非变性聚丙烯酰胺凝胶电泳。结果表明,有5对引物扩增效果较好,可用于长尾仓鼠的遗传多样性研究。研究结果可对今后长尾仓鼠的遗传多样性分析提供一定的理论基础。In this study,the genomic DNA was extracted from 132 Cricetulus longicaudatus,which was collected from 13 counties(cities)in Shanxi province.Six pairs SSR primers of Cricetulus longicaudatus were used to amplify the genomic DNA by PCR.The amplified product was subjected in 8%non-denaturing polyacrylamide gel electrophoresis.The results showed that five pairs of primers had good amplification effect and could be used to study the genetic diversity of Cricetulus longicaudatus.This study result will provide a theoretical basis for the genetic diversity analysis of the Cricetulus longicaudatus.

关 键 词：长尾仓鼠 微卫星DNA PCR 非变性聚丙烯酰胺凝胶电泳 

分 类 号：S443.3[农业科学—农业昆虫与害虫防治]