葡萄籽原花青素提取物对心肌梗死大鼠心肌功能的影响及其机制研究  

Effects of GSPE on myocardial function in rats with acute myocardial infarction and its possible mechanisms

作　　者：张中喜[1] 曾令红 陈永衡[2] 高国应 邹平 ZHANG Zhong-xi;ZENG Ling-hong;CHEN Yong-heng(Department of Ultrasound,The First Affiliated Hospital of Changsha Medical College,Changsha Hunan 410219,China;Department of Cardiology,The First Affiliated Hospital of Changsha Medical College,Changsha Hunan 410219,China)

机构地区：[1]长沙医学院第一附属医院超声科,湖南长沙410219 [2]长沙医学院第一附属医院心内科,湖南长沙410219 [3]长沙医学院第一附属医院中西医结合科,湖南长沙410219

出　　处：《临床和实验医学杂志》2019年第20期2157-2160,共4页Journal of Clinical and Experimental Medicine

基　　金：湖南省教育厅科学研究项目(编号:16C0165)

摘　　要：目的研究葡萄籽原花青素提取物(GSPE)对心肌梗死大鼠心功能的影响及其可能机制。方法取48只SD大鼠,其中40只用于建立心肌梗死大鼠模型,并随机分为模型组、GSPE低剂量组、GSPE中剂量组、GSPE高剂量组及BSI-201组,每组各8只,余8只大鼠为假手术对照组。模型组与对照组均给予等量生理盐水灌胃,GSPE低剂量组采用100 mg/ml GSPE进行灌胃给药,GSPE中剂量组采用200 mg/ml GSPE进行灌胃给药,GSPE高剂量组给予400 mg/ml GSPE进行灌胃给药,BSI-201组采用多聚腺苷酸二磷酸核糖聚合酶-1(PARP-1)抑制剂BSI-201 120 mol/L进行灌胃给药,1次/d,持续1个月。采用彩色多普勒超声心动图评估各组大鼠心功能状况,记录心率(HR)、左心室舒张末压(LVEDP)及左心室收缩压(LVSP)。通过苏木精-伊红染色评估各组大鼠心肌组织病理学改变状况,采用TUNEL法对大鼠心肌细胞凋亡情况进行检测,并用蛋白免疫印迹法检测大鼠心肌组织中PAR、切割的Caspase-3(c-Caspase-3)、Bcl-2及Bax蛋白的表达水平。结果相比对照组,模型组大鼠LVSP明显下降,HR、LVEDP及细胞凋亡率明显升高(P<0.05),心肌纤维排列不一,心肌细胞破碎,出现大量炎性细胞浸润;心肌组织中Bcl-2蛋白表达水平显著下降,PAR、c-Caspase-3及Bax蛋白的表达水平明显升高(P<0.05)。相比模型组,GSPE低、中、高剂量组和BSI-201组大鼠LVSP明显升高,HR、LVEDP及细胞凋亡率显著下降(P<0.05),心肌组织病理损伤程度明显减轻;心肌组织中Bcl-2蛋白表达水平显著升高,PAR、c-Caspase-3及Bax蛋白的表达水平明显下降(P<0.05),并呈剂量依赖性。结论GSPE可呈剂量依赖性有效改善心肌梗死大鼠心脏功能,其作用机制可能与阻滞PARP-1活化以起到阻滞心肌细胞凋亡的作用密切相关。Objective To study the effect of grape seed proanthocyanidin extract(GSPE)on cardiac function in rats with acute myocardial infarction and its possible mechanism.Methods Forty-eight SD rats were randomly divided into acute myocardial infarction model group,low-dose GSPE group,middle-dose GSPE group,high-dose GSPE group and BSI-201 group,with eight rats in each group,and eight rats were used as sham-operated control group.The model group and the control group were given the same amount of saline by gavage,the low-dose group was given 100 mg/ml GSPE by gavage,the middle-dose group was given 200 mg/ml GSPE by gavage,the high-dose group was given 400 mg/ml GSPE by gavage,and the BSI-201 group was given BSI-120 mol/L,a PARP-1 inhibitor,1 time/day,lasted for one month.Cardiac function was assessed by color Doppler echocardiography.Heart rate(HR),left ventricular end diastolic pressure(LVEDP)and left ventricular systolic pressure(LVSP)were recorded.Myocardial histopathological changes were assessed by hematoxylin-eosin staining.Apoptosis of myocardial cells was detected by TUNEL method.PAR,cleaved Caspase-3(c-Caspase-3),Bcl-2 and Bax protein were detected by Western blot.Results Compared with the control group,LVSP in the model group decreased significantly,HR,LVEDP and apoptotic rate increased significantly(P<0.05),myocardial fibers arranged differently,myocardial cells were broken and a large number of inflammatory cells infiltrated;Bcl-2 protein expression in myocardial tissue decreased significantly,and PAR,c-Caspase-3 and Bax protein expression levels increased significantly(P<0.05).Compared with the model group,the LVSP of rats in low,medium and high dose GSPE group and BSI-201 group increased significantly,the HR,LVEDP and apoptotic rate decreased significantly(P<0.05),the degree of myocardial pathological injury decreased significantly,the expression of Bcl-2 protein in myocardial tissue increased significantly,and the expression levels of PAR,c-Caspase-3 and Bax protein decreased significantly(P<0.05).Conclusio

关 键 词：大鼠 心肌梗死 葡萄籽原花青素 心功能 

分 类 号：R28[医药卫生—中药学]