乙酸钠丙酸钠配比对A^2/O-BCO反硝化除磷及菌群结构的影响  

Effect of the ratios of sodium acetate to sodium propionate on denitrifying phosphorus removal characteristics in the A^2/O-BCO process

作　　者：於蒙 潘婷 张淼[1] 王一鑫 庞晶津 姜友法[3] 王宝林[3] 何成达[1] YU Meng;PAN Ting;ZHANG Miao;WANG Yi-xin;PANG Jing-jin;JIANG You-fa;WANG Bao-lin;HE Cheng-da(College of Environmental Science and Engineering,Yangzhou University,Yangzhou 225127,China;Yangzhou Jieyuan Drainage Company Limited,Yangzhou 225002,China;Jiangsu Yangnong Chemical Company Limited,Yangzhou 225009,China)

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225127 [2]扬州市洁源排水有限公司,江苏扬州225002 [3]江苏扬农化工股份有限公司,江苏扬州225009

出　　处：《中国环境科学》2019年第10期4178-4185,共8页China Environmental Science

基　　金：国家自然科学基金资助项目(51808482);江苏省自然科学基金资助项目(BK20170506);博士后科学基金资助面上项目(2018M632392);江苏省水环境保护技术与装备工程实验室开放课题(W1803)

摘　　要：采用厌氧/缺氧/好氧和生物接触氧化反应器(A2/O-BCO)组成的反硝化除磷系统处理模拟生活污水,通过调节进水乙酸钠、丙酸钠的配比(乙酸钠:丙酸钠分别为1:0,2:1,1:1,1:2和0:1),考察了系统对有机物的去除以及同步脱氮除磷的影响,同时通过高通量测序对比了不同配比下微生物菌群结构的变化.结果表明:乙酸钠丙酸钠配比对有机物和NH4+-N的去除影响较小,对厌氧段有机物的消耗和TN的去除率以及磷的释放和吸收影响较为明显;TP去除率仅为50.3%~56.8%,需进一步优化系统的运行参数.当乙酸钠:丙酸钠=1:1时,厌氧段有机物消耗量最大,占有机物流入量的61.2%,厌氧释磷量最大(23.2mg/L)且缺氧吸磷率最高(71.4%),而TN的去除效果则随丙酸钠含量的增加而增加.高通量测序结果表明:A2/O反应器中微生物多样性降低,混合碳源污泥中微生物多样性比单一碳源更丰富;驯化后的污泥中绿弯菌(Chloroflexi)和螺旋菌(Saccharibacteria)减少,变形菌(Proteobacteria)和拟杆菌(Bacteroidetes)增加.BCO反应器中Nitrospira和Nitrosomonas总占比为2.1%~31.4%,且抑制亚硝酸盐氧化菌(NOB)的活性,有利于短程硝化的实现.A denitrifying phosphorus removal system combining anaerobic/anoxic/oxic(A2/O)with biological contact oxidation process(BCO)was used to treat synthetic wastewater.By adjusting the ratio of sodium acetate to sodium propionate(sodium acetate:sodium propionate=1:0,2:1,1:1,1:2 and 0:1),the organic matter removal and denitrifying phosphorus removal characteristics of the system were investigated.The results showed that the ratio of sodium acetate to sodium propionate revealed a negligible effect on organic matter and NH4+-N removals,but the influences on organic consumption in the anaerobic stage,total nitrogen removal as well as the phosphorus release and absorption were obvious.The operation parameters of the system need to be further optimized due to the low phosphorus removal of 50.3%~56.8%.When the sodium acetate/sodium propionate was 1:1,the organic matter consumption in the anaerobic section was the highest,accounting for 61.2%of the total organic matter input,with the maximum anaerobic phosphorus releaseamount(23.2mg/L)and anoxic phosphorus uptake rate(71.4%).However,the TN removal increased with the addition of sodium propionate.High-throughput sequencing results showed that the microbial diversity in the A2/O reactor was lower,while the mixed carbon source sludge was more abundant than that of the single carbon source.Particularly,the amount of Chloroflexi and Saccharibacteria reduced while the Proteobacteria and Bacteroidetes increased in the domesticated sludge.The total proportion of nitrifying bacteria in the BCO reactor was 2.1%~31.4%,and the ammonia oxidizing bacteria(AOB)was dominate which favoured the realization of short-cut nitrification.

关 键 词：A2/O-BCO系统 碳源 反硝化除磷 高通量测序 微生物菌群结构 

分 类 号：X703.1[环境科学与工程—环境工程]