不同异交率大豆细胞质雄性不育系的转录组分析  

Transcriptomic analysis of soybean cytoplasmic male sterile lines with different outcrossing rate

作　　者：李蓉[1,2,3] 林春晶 彭宝[2,3] 丁孝羊 李永宽[1,2,3] 赵国龙 赵丽梅 张春宝 LI Rong;LIN Chun-jing;PENG Bao;DING Xiao-yang;LI Yong-kuan;ZHAO Guo-long;ZHAO Li-mei;ZHANG Chun-bao(Agricultural College,Jilin Agricultural University,Changchun 130018,China;Soybean Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033,China;National Engineering Research Center of Soybean,Changchun 130033,China)

机构地区：[1]吉林农业大学农学院,吉林长春130018 [2]吉林省农业科学院大豆研究所,吉林长春130033 [3]大豆国家工程研究中心,吉林长春130033

出　　处：《中国油料作物学报》2019年第5期696-704,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31971969);吉林省科技发展计划资助项目(20190101007JH;20170201001NY);吉林省农业科技创新工程项目(c82230412)

摘　　要：不育系异交结实率在杂交制种中起着重要作用。本研究以异交率差异显著的两个大豆细胞质雄性不育系材料为研究对象,在盛花期对其成熟花苞进行转录组测序。经比较分析,筛选出1925条差异基因,其中1361条差异基因注释到GO分类的生物过程、细胞组分和分子功能3大类30个分支中,主要涉及生物过程、催化活性、氧化还原酶活性、氧化还原过程和碳水化合物代谢过程。864条差异基因注释到KEGG分类的114个代谢通路中,主要包括代谢途径通路、次生代谢物的生物合成通路以及植物激素信号转导通路,其中包括13个次生代谢通路,主要为苯丙烷类生物合成以及类黄酮生物合成。通过对差异显著、富集基因数目较多的类黄酮生物合成途径进行分析,进一步挖掘类黄酮生物合成途径中差异表达基因,筛选到影响花色的基因FLS1,推测花色会影响昆虫对花粉的传播,从而影响异交率。本研究期望有助于解析影响大豆异交率的生物合成通路及明确异交率分子机制。The outcrossing rate of sterile lines plays an important role in hybrid seed production.In this study,two soybean cytoplasmic male sterile lines with significant difference in outcrossing rate were studied,and their ma ture flower buds at full bloom stage were tested.Transcriptome sequencing was performed.After comparative analy sis,1925 differential genes were screened out.Based on GO classification,1361 differential genes were annotated into the 30 branches of the biological processes,cell components and molecular functions,main biological process es,catalytic activities,oxidoreductase activities,redox process and carbohydrate metabolism process.864 differen tial genes were annotated into 114 metabolic pathways by KEGG classification,including metabolic pathway path ways,biosynthetic pathways of secondary metabolites,and phytohormone signal transduction pathways,including 13 secondary metabolic pathways,main phenylpropanoid biosynthesis and flavonoid biosynthesis.According to sig nificant value of gene difference and the number of enriched genes,the pathways of flavonoid biosynthesis were ana lyzed,gene FLS was screened as the key to flower color.Previous report showed the flower color affected induce ment insect to transfer pollen,which further influenced outcrossing rate.This study was to elucidate the biosynthetic pathways affecting soybean outcrossing rate and clarify the molecular mechanism of soybean outcrossing rate.

关 键 词：大豆 细胞质雄性不育系 异交率 转录组测序 代谢通路 

分 类 号：S330[农业科学—作物遗传育种]