镁合金微弧氧化掺杂Zn-HA复合涂层细胞相容性与抗菌性  

Cellular Compatibility and Antibacterial Properties of Magnesium Alloy after Ultrasonic Microarc Oxidation-doped ZnHA Silane-phytic Acid Treatment

作　　者：张鹏 周立波[1] 张慧明[2] 张美玲 姜枫 黄宇博 李慕勤 李德超[1] ZHANG Peng;ZHOU Libo;ZHANG Huiming;ZHANG Meiling;JIANG Feng;HUANG Yubo;LI Muqin;LI Dechao(Stomatological Hospital,Jiamusi University,Jiamusi 154007,China;Life Science Center,Jiamusi University,Jiamusi 154007,China;Key Laboratory of College of Biomedical Materials of Heilongjiang Province,Jiamusi University,Jiamusi 154007,China)

机构地区：[1]佳木斯大学附属口腔医院,黑龙江佳木斯154007 [2]佳木斯大学生命科学中心,黑龙江佳木斯154007 [3]黑龙江省高校生物医学材料重点实验室,黑龙江佳木斯154007

出　　处：《口腔医学研究》2019年第10期1001-1004,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:31370979);黑龙江省卫生计生委科研课题(编号:2018037);佳木斯大学口腔病防治协同创新中心(编号:2011xtcx2016-02)

摘　　要：目的:通过掺杂Zn-HA复合涂层处理调节镁合金的生物活性和增加抗菌性。方法:以镁合金微弧氧化硅烷-植酸复合膜为对照组,添加2 g/L、4 g/L、6 g/L Zn-HA为实验组,通过扫描电镜、CCK-8试剂盒、激光共聚焦显微镜等方式检测各组膜层生物相容性,采用吖啶橙/溴化乙锭(AO/EB)双重荧光染色法检测抗菌性。结果:CCK-8粘附与增殖实验测定的吸光率结果均为C组>D组>B组>A组;激光共聚焦显微镜显示载4 g/L Zn-HA组试件表面细胞伸展最好,载6 g/L Zn-HA后涂层的抗菌性优于其他各组。结论:镁合金微弧氧化掺杂4 g/L Zn-HA硅烷-植酸处理后膜层的生物相容性最好,镁合金微弧氧化掺杂6 g/L Zn-HA硅烷-植酸处理后膜层具有较强抗菌能力。Objective:To test the cellular biocompatibility and antibacterial property of magnesium alloy after ultrasonic microarc oxidation-doped Zn-HA silane-phytic acid treatment.Methods:The magnesium alloy UMAO-silane-phytic was designed as the control group.Zn-HA at concentration of 2 g/L,4 g/L,and 6 g/L were added respectively as the experimental group.The compatibility of different treatment membrane cells was detected by CCK-8 kit,scanning electron microscope,and laser confocal microscope.Acridine orange/ethidium bromide(AO/EB)double fluorescent staining was used to detect the antibacterial.Results:The optical density(OD)value of cell adhesion and proliferation measured by CCK-8.The OD values were in the order at:group C>group D>group B>group A.The number and shape of cells in the group C had the best extension.The antibacterial of 6 g/L Zn-HA group was obviously superior to other groups.Conclusion:The UMAO-doped 4 g/L Zn-HA silane-phytic acid treatment of magnesium alloy had the best biocompatibility.The UMAO-doped 6g/L Zn-HA silane-phytic acid treatment of magnesium alloy had the strongest antibacterial ability.

关 键 词：镁合金 超声微弧氧化 Zn-HA 硅烷 植酸 细胞相容性 抗菌性 

分 类 号：TG1[金属学及工艺—金属学]