钯(Ⅱ)-吡啶二羧酸-氨基酸配合物的合成及其与DNA的相互作用  

Synthesis and DNA Binding Studies for the Complex of Pd（Ⅱ）-H2pydc-Amino Acid

作　　者：陈佳奇 宋振峰 杨海霞 孙薪博 冯文爽 高恩军 CHEN Jia-qi;SONG Zhen-feng;YANG Hai-xia;SUN Xin-bo;FENG Wen-shuang;GAO En-jun(Shenyang University of Chemical Technology,Shenyang 110142,China)

机构地区：[1]沈阳化工大学辽宁省无机分子基化学重点实验室

出　　处：《沈阳化工大学学报》2019年第3期193-198,共6页Journal of Shenyang University of Chemical Technology

基　　金：国家自然科学基金(21171118)

摘　　要：在常温下用四氯化钯酸钾和吡啶-2,3-二羧酸(2,3-H 2pydc)、2-氯-L-苯丙氨酸(L-2-Cl-Phe)反应制得新颖的混配配合物[Pd(2,3-H 2pydc)(L-2-Cl-Phe)],通过核磁共振1H-NMR对配合物结构进行验证,应用荧光光谱法及紫外光谱法对该配合物与小牛胸腺DNA(CT-DNA)的结合方式进行研究.研究结果证明:该配合物与DNA的结合方式以插入结合方式为主.与此同时,通过凝胶电泳实验证明该配合物对质粒DNA(pBR322-DNA)的切割能力.A novel complex[Pd(2,3-H 2pydc)(L-2-Cl-Phe)]was obtained by the reaction of 2,3-H 2pydc and L-2-Cl-Phe at room temperature.The structure of the complex was verified by 1H-NMR.The combination mode of the complex with calf thymus DNA(CT-DNA)was characterized by fluorescent spectrometry and ultraviolet spectroscopy.The results showed that the combination of the complex and DNA was mainly by insertion.Meanwhile,the cleavage ability of the complex to plasmid DNA(pBR322-DNA)was confirmed by gel electrophoresis experiment.

关 键 词：钯(Ⅱ)配合物 紫外光谱法 荧光光谱法 凝胶电泳 切割能力 结合方式 

分 类 号：TQ138.2[化学工程—无机化工]