羊口疮病毒在GT26细胞系中的增殖特性研究  

Study on Proliferative Characteristics of ORFV in GT26 Cell Line

作　　者：刘阿华 王毅 李言言 高洋 谷奎 马文涛 陈德坤[1] LIU A-Hua;WANG Yi;LI Yan-yan;GAO Yang;GU Kui;MA Wen-tao;CHEN De-kun(College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi,712100,China)

机构地区：[1]西北农林科技大学动物医学院

出　　处：《动物医学进展》2019年第10期23-27,共5页Progress In Veterinary Medicine

基　　金：青海省重点研发与转化计划项目(2018-NK-125);咸阳市重大科技项目(2017K01-34)

摘　　要：为了明确羊口疮病毒(ORFV)在GT26细胞系上的增殖特性,将ORFV分别接种于GT26细胞系、羊睾丸原代细胞(GTC)、牛睾丸原代细胞(BTC)和山羊胎儿皮肤成纤维细胞(GFFC)。比较上述细胞的细胞病变(CPE)特点并进行PCR检测和TCID 50测定,从而评估GT26增殖ORFV的效果。结果显示,ORFV能在GT26细胞上增殖,并能产生明显的CPE,接毒后36 h开始出现CPE,72h CPE更加明显;与其他3种原代细胞一样,在PCR检测中,接种了ORFV的GT26细胞裂解液中能扩增出540 bp的目的片段;ORFV在GT26细胞的TCID 50为10-6.5,而在BTC、GFFC和GTC细胞的TCID 50分别为10-6.7、10-6.3、10-7.0。表明ORFV在GT26细胞上的滴度与上述3种原代细胞相当,GT26细胞能够用于大量增殖ORFV。The proliferation characteristics of ORFV in GT26 cell line was studied by infecting GT26 cells,goat primary testicular cells(GTC),bovine primary testicular cells(BTC)and goat fetal fibroblast cells(GFFC).Then,CPE,PCR,and TCID 50 detection were used to evaluate the effect of proliferation of ORFV in GT26 cells.The results showed:after infection with ORFV,CPE was appeared at 36h and increased at 72 h;In the PCR assay,the 540 bp target fragment was amplified in all the above infected cells;The TCID 50 of ORFV in GT26 cells was basically the same as the three primary cells mentioned above.In summary,GT26 cells could be used to proliferate ORFV in a large amount.

关 键 词：羊口疮病毒 GT26细胞系 原代细胞 增殖特性 

分 类 号：S852.659.6[农业科学—基础兽医学]