广西环江喀斯特常绿落叶阔叶林土壤微生物类群的空间格局  

Spatial pattern of soil microbial taxa in a karst evergreen and deciduous broadleaf forest in Huangjiang of Guangxi

作　　者：宋敏[1,2,3] 彭晚霞[2,3] 徐庆国[1] 杜虎[2,3] SONG Min;PENG Wanxia;XU Qingguo;DU Hu(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China;Huanjiang Observation and Research Station for Karst Ecosystems,Chinese Academy of Sciences,Huanjiang,Guangxi 547100,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室,湖南长沙410125 [3]中国科学院环江喀斯特生态系统观测研究站,广西环江547100

出　　处：《湖南农业大学学报（自然科学版）》2019年第5期507-513,共7页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：国家重点研发计划项目(2016YFC0502405);国家自然科学基金项目(31770495);广西科学研究与技术开发计划项目(桂科转1599001–6，桂科2016AB12095)

摘　　要：运用地理统计学和Illumina 16S rRNA基因测序等手段,研究广西环江木论国家自然保护区25 hm2喀斯特常绿落叶阔叶林土壤主要门级微生物类群的空间分布。结果表明:广西环江木论国家自然保护区喀斯特森林土壤中优势菌门为变形菌门(Protebacteria,34.5%)、放线菌门(Actinobacteria,30.7%)和酸杆菌门(Acidobacteria,12.2%);土壤细菌类群显示空间相关性的自相关范围为44.4~841.4 m,其中大部分细菌类群的变程在研究区域范围(500 m)内;在研究区内,变形菌门的相对丰度从北到南逐渐升高,而放线菌门和厚壁菌门(Firmicutes)的相对丰度则从北到南逐渐降低,绿弯菌门(Chloroflexi)、酸杆菌门、奇古菌门(Thaumarchaeota)、拟杆菌门(Bacteroidetes)、芽单胞菌门(Gemmatimonadetes)、疣微菌门(Verrucomicrobia)的相对丰度呈斑片状分布,硝化螺旋菌门(Nitrospirae)的相对丰度呈单峰型分布,WS3(Latescibacteria)的相对丰度则呈间歇性的低值条带和高值条带状分布。可见,喀斯特土壤主要微生物类群相对丰度呈现不同的空间分布格局。To understand the spatial pattern of soil microbial communities at the phyla level in Mulun National Natural Reserve in Huangjiang of Guangxi,we used geostatistical modeling and Illumina sequencing of 16S rRNA genes to the soils in a 25 hm2 karst evergreen deciduous broadleaf forest.The results showed that the dominant bacterial phyla in the karst forest soils were Protebacteria(34.5%),Actinobacteria(30.7%)and Acidobacteria(12.2%).Soil bacterial taxa showed spatial dependence with an autocorrelation range of 44.4-841.4 m,and most of them were within the scope of the study plot(500 m).An increasing trend was observed for Proteobacteria from north to south in the studied area,but an opposite trend for Actinobacteria and Firmicutes was observed.The spatial pattern for Chloroflexi,Acidobacteira,Thaumarchaeota,Bacteroidetes,Gemmatimonadetes and Verrucomicrobia could be characterized as patchy,and the spatial pattern for Nitrospirae could be unimodal.In addition,the spatial pattern for Latescibacteria could be intermittent with low and high value strips.Overall,our results demonstrated that the spatial distribution of soil microbial communities differs among various taxa in karst forest.

关 键 词：土壤微生物群落 常绿落叶阔叶林 空间格局 Illumina测序 喀斯特 木论国家自然保护区 

分 类 号：S154.36[农业科学—土壤学]