沼液浸种对蒙古黄芪种子萌发及幼苗生理特性的影响  

Effects of biogas slurry soaking seeds on seed germination and physiological characteristics of Astragalus membranaceus var. mongholicus seedling

作　　者：陆国弟[1] 杨扶德[1] 王惠珍[1] 杜弢[1] 郑健[2] 邢豪 LU Guo-di;YANG Fu-de;WANG Hui-zhen;DU Tao;ZHENG Jian;XING Hao(Gansu University of Chinese Medicine,Lanzhou 730000;Lanzhou University of Technology,Lanzhou 730050)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]兰州理工大学,甘肃兰州730050

出　　处：《中国土壤与肥料》2019年第5期155-162,共8页Soil and Fertilizer Sciences in China

基　　金：甘肃省科技厅青年项目(177R5RA166);甘肃省教育厅创新能力提升项目(2019B-102);西北中藏药创新中心2015年度重点项目(XBXT2015-4)

摘　　要：通过培养皿发芽试验,设计5个沼液浓度水平(0%,10%,25%,50%,75%)和3个浸种时间水平(4,5,6 h),探讨沼液浓度和浸种时间对蒙古黄芪种子发芽势、发芽指数、活力指数、根长、苗高、根重、叶绿素(Chl)含量、丙二醛(MDA)含量、抗氧化酶活性(CAT、POD、SOD)及根系活力的影响,以寻求最佳沼液浸种浓度和时间组合,为黄芪产量和质量的提高提供理论依据。结果表明,25%沼液浸种5 h黄芪种子发芽势最高,显著高于清水浸种4、5 h处理(P<0.05);25%沼液浸种5 h的发芽指数、活力指数、根长、根重,Chla、Chlb、Chla+Chlb含量,根系活力、POD活性、SOD活性均显著高于其他14个浸种处理(P<0.05);25%沼液浸种4、6 h苗高均显著高于其余13个处理(P<0.05);丙二醛含量与CAT活性测定结果一致,均以25%沼液浸种5 h最低(P<0.05)。说明适宜的沼液浸种有利于黄芪种子萌发和幼苗生长。其中25%沼液浸种5 h对黄芪种子萌发和幼苗生长效果最佳。The influences of different biogas slurry concentrations and soaking time on the seed germination and physiological characteristics of Astragalus membranaceus var.mongholicus seedling were studied by the culture dish germination experiment.And germination power,germination index,vigor index,root and seedling length,root height,content of chlorophyll and MDA,antioxidant enzyme activities and root activity in various biogas slurry concentrations(0%,10%,25%,50%,75%)and soaking time(4,5,6 h)were recorded and analyzed with PCA analysis,and the best biogas slurry soaking concentration and time were explored to improve the yield and quality of Astragalus membranaceus var.mongholicus.The results showed that,the germination power of seeds was the highest in 25%biogas slurry soaking for 5 h,which was significantly higher than that in water soaking for 4 and 5 h(P<0.05).The germination index,vigor index,root length,root weight,Chla content,Chlb content,Chla+Chlb content,root activity,POD and SOD activity of seeds under 25%biogas slurry soaked for 5 h were significantly higher than the other 14 soaking treatments(P<0.05).The seedling height of seeds under 25%biogas slurry soaked for 4 and 6 h was the highest(P<0.05).MDA content and CAT activity of seeds under 25%biogas slurry soaked for 5 h were the lowest(P<0.05).It can be concluded that appropriate biogas slurry soaking can improve the germination and growth of Astragalus membranaceus var.mongholicus.As a whole,soaking for 5 h in 25%biogas slurry had the best effect on seed germination and growth of Astragalus membranaceus var.mongholicus seedling.

关 键 词：沼液浓度 浸种时间 蒙古黄芪 种子萌发 幼苗生理特性 

分 类 号：S51[农业科学—作物学]