不同日龄鸡肺脏中CD4^+、CD8^+ T淋巴细胞分布规律  

The Localization Changes of CD4^+ and CD8^+ T Lymphocytes in Chicken Lung at Different Ages

作　　者：张英楠 徐晶 栾维民[2] 杨树宝[2] ZHANG Yingnan;XU Jing;LUAN Weimin;YANG Shubao(Changchun University of Science and Technology,Changchun 130600,China;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]长春科技学院,长春130600 [2]吉林农业大学动物科技学院,长春130118

出　　处：《中国畜牧兽医》2019年第10期3052-3057,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金项目(31372391)

摘　　要：为了解鸡肺脏不同时期的免疫状态,选择不同日龄海兰白鸡的肺脏组织,利用免疫组织化学方法研究CD4^+和CD8^+T淋巴细胞的出现、定位分布及数量变化过程。结果显示,CD8^+细胞最初于18 d胚胎中出现,而CD4^+T淋巴细胞于孵化后1日龄雏鸡中出现。4日龄时,在初级支气管与次级支气管交汇处有淋巴细胞的聚集物,即形成了明显的支气管相关性淋巴组织(BALT)。在定位分布上,各日龄CD4^+T淋巴细胞主要占据BALT的中央区域,而CD8^+T淋巴细胞主要环绕在外周。从56日龄开始,CD8^+T细胞较CD4^+T细胞分布范围更为广泛,在三级支气管气道内壁中、房间隔中、气体交换区和小叶间结缔组织中均有分布,散布在整个肺脏中。在数量变化上,随着日龄增长,CD4^+T淋巴细胞和CD8^+T淋巴细胞数量均逐渐增加,且35日龄之前CD4^+T细胞数量多于CD8^+T细胞,而此后各日龄CD8^+T细胞的数量均显著多于CD4^+细胞。以上结果表明,鸡肺脏中CD4^+和CD8^+T淋巴细胞的分布和数量均呈日龄相关性变化,且该变化说明鸡35日龄之前肺脏以体液免疫为主,而之后则倾向于细胞免疫。The aim of this study was to investigate the immune state of chicken lung in different periods.With lung tissue of Hy-line White chicken at different ages,the distribution and quantity changes of CD4^+and CD8^+T lymphocytes in lung were studied using immunohistochemistry staining.The results showed that CD8^+T lymphocytes appeared firstly in embryonic at 18 d,while CD4^+T lymphocytes appeared at 1-day-old chicken.At 4-day-old,there were aggregates of lymphocytes at the junction of the primary and secondary bronchi,which formed obvious broncho-associated lymphoid tissue(BALT).CD4^+T lymphocytes of each age mainly occupied the central area of BALT,while CD8^+T lymphocytes mainly surrounded the periphery.Since 56 days old,CD8^+T lymphocytes are distributed in the inner wall of third-order bronchial airway,atrial septum,gas exchange area and interlobular connective tissue,and are distributed throughout the lung.In terms of quantity change,with the growth of daily age,the number of CD4^+T lymphocytes and CD8^+T lymphocytes gradually increased,and the number of CD4^+T lymphocytes was more than that of CD8^+T lymphocytes before 35 days of age,while the number of CD8^+T lymphocytes was significantly more than that of CD4^+T lymphocytes at the same age thereafter.The results showed that the distribution and number of CD4^+and CD8^+T lymphocytes in the lungs of chickens were correlated with the age,and the changes could reflect that the lungs before the age of 35 days were dominated by humoral immunity,while the lungs after that tended to be cellular immunity.

关 键 词：鸡 肺脏 CD4^+T淋巴细胞 CD8^+T淋巴细胞 分布 

分 类 号：S852.4[农业科学—基础兽医学]