紫锥菊多糖与硫酸化紫锥菊多糖颉颃环磷酰胺对雏鸡免疫抑制作用的研究  

Study on Antagonistic Effects of Echinacea purpurea Polysaccharide and Sulfated Echinacea purpurea Polysaccharide on Immunosuppression of Cyclophosphamide in Chickens

作　　者：姚丽丽 白霖 谭盈 孙嘉琪 石达友 郭世宁 刘翠 YAO Lili;BAI Lin;TAN Ying;SUN Jiaqi;SHI Dayou;GUO Shining;LIU Cui(Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Nature Medicine,College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学兽医学院广东省兽用中药与天然药物工程技术研究中心

出　　处：《中国畜牧兽医》2019年第10期3084-3094,共11页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31602096、31672594);广东省财政技术研究与开发补助费用项目([2015]639)

摘　　要：试验旨在研究紫锥菊多糖(EPP)与硫酸化紫锥菊多糖(SEPP)对雏鸡免疫抑制状态的调节作用,将320羽14日龄健康岭南黄公鸡随机均分成空白组(NC)、环磷酰胺组(CY)及CY+EPP高、中、低3个剂量组(EPPH、EPPM和EPPL组)和CY+SEPP高、中、低3个剂量组(SEPPH、SEPPM和SEPPL组),除NC组注射生理盐水外,其余7组连续3 d肌内注射80 mg/(kg·d)CY进行造模,造模后EPPH、EPPM和EPPL组连续7 d分别给予10、5、2.5 mg/(kg·d)EPP,SEPPH、SEPPM和SEPPL组连续7 d分别给予10、5、2.5 mg/(kg·d)SEPP。在首次给药后第7、14、21和28天测定鸡群的体重,肝脏、脾脏、肾脏的脏器指数,外周血淋巴细胞转化作用,血液生化指标,细胞因子和抗氧化因子指标。结果表明,SEPP和EPP均能显著提高CY诱导免疫抑制鸡体重、肝脏指数、脾脏指数和肾脏指数(P<0.05),提高外周血淋巴细胞转化作用,降低葡萄糖(GLU)、尿素(UREA)浓度和碱性磷酸酶(ALP)活性,升高谷草转氨酶(AST)、白蛋白(ALB)浓度,且能显著提高白细胞介素-2(IL-2)、IL-6浓度(P<0.05),SEPP效果优于EPP。说明雏鸡口服一定剂量的EPP和SEPP对CY引起的细胞免疫和体液免疫抑制具有一定的颉颃作用,其中在各采样时间点SEPP对鸡外周血淋巴细胞转化效果优于EPP,给药21和28 d SEPP对鸡外周血细胞因子IL-2的分泌效果优于EPP。The aim of this study was to investigate the effects of Echinacea purpurea polysaccharide(EPP)and sulfated Echinacea purpurea polysaccharide(SEPP)on immunosuppressive status in chickens.320 14-day-old healthy Lingnan Yellow cocks were randomly divided into blank group(NC),cyclophosphamide group(CY),CY+EPP high,medium and low dose group(EPPH,EPPM and EPPL groups)and CY+SEPP high,medium and low dose group(SEPPH,SEPPM and SEPPL groups).Except NC group,the other 7 groups were injected 80 mg/(kg·d)CY intramuscularly for 3 days.EPPH,EPPM and EPPL groups were administered 10,5,2.5 mg/(kg·d)EPP for 7 consecutive days.SEPPH,SEPPM and SEPL groups were administered 10,5,2.5 mg/(kg·d)SEPP for 7 consecutive days.The body weight,organ indexes of liver,spleen and kidney,transformation of peripheral blood lymphocyte,blood biochemical index,cytokine and antioxidant factor were measured on the 7th,14th,21st and 28th day after the first administration.The results showed that SEPP and EPP could significantly increase the body weight,liver index,spleen index and kidney index of immunosuppressive chickens induced by CY(P<0.05),as well as the transformation of peripheral blood lymphocyte,decrease the concentration of glucose(GLU),urea(UREA)and alkaline phosphatase(ALP),and increase the activity of aspartate aminotransferase(AST).Concentrations of albumin(ALB)and interleukin-2(IL-2),IL-6 were significantly increased(P<0.05).SEPP was better than EPP.The results showed that oral dose of EPP and SEPP could antagonize the cellular and humoral immunosuppression induced by CY in chickens.SEPP had better effect on lymphocyte transformation in peripheral blood of chickens at different sampling time points than EPP,and SEPP had better effect on IL-2 secretion in peripheral blood of chickens at 21 and 28 days than EPP.

关 键 词：紫锥菊多糖(EPP) 硫酸化紫锥菊多糖(SEPP) 环磷酰胺(CY) 免疫抑制 外周血淋巴细胞转化作用 血液生化指标 细胞因子 

分 类 号：R285.5[医药卫生—中药学]