转录因子FOSB mRNA 5′非翻译区内部核糖体进入位点的活性  

Activity of Internal Ribosme Entry Site in m RNA 5′ Untranslation Region of Transcription Factor FOSB

作　　者：马晶 孙柳柳 马文囡 陶义芬 陈蕴 朱瑞宇 金坚 MA Jing;SUN Liuliu;MA Wennan;TAO Yifen;CHEN Yun;ZHU RuiYu;JIN Jian(School of Pharmaceutical Sciences,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学药学院

出　　处：《食品与生物技术学报》2019年第7期41-48,共8页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目（81101667）

摘　　要：研究FOSB基因侧翼序列是否含有内部核糖体进入位点(internal ribosme entry site,IRES),进而探究其生理功能。作者利用双顺反子报告载体(pRL-FL),构建检测质粒(pRL-FOSB-FL),并将其转染到人胚肾细胞HEK293和4种癌细胞中,检测FOSB mRNA 5′非翻译区(5′untranslated region,5′UTR)IRES活性并通过逐步截断探索其活性中心,最后对肝癌细胞Bel-7402进行药物(紫杉醇和顺铂)刺激。报告载体的结果显示,FOSB mRNA 5′UTR具有IRES活性,且在不同细胞中FOSB IRES活性存在显著性差异,在卵巢癌细胞A2780/WT细胞中FOSB IRES活性较高。序列截断发现,FOSB mRNA 5′UTR中的160nt(-376到-217)对其IRES的功能至关重要.在肝癌细胞中,随着加药浓度的增加,其IRES活性逐渐增加,尤其在紫杉醇刺激下活性增加更为明显。本研究发现的FOSB基因的这些特性为研究FOSB在肿瘤的预防与治疗提供了一种新的思路。To explore the possible sequence and function of internal ribosme entry site(IRES)in flanking sequence of FOSB.Using the typical bicistronic reporter system of dual-luciferase,constructing the reporter plasmids containing the 5'UTR of FOSB mRNA and then transfecting them into HEK293 cell and 4 strains of cancer cells.Performing the deletion of FOSB mRNA 5'UTR and stimulating the Bel-7402 cell with anticancer drug,paclitaxel or cis-platinum.The results showed that the FOSB mRNA 5'UTR has IRES activity and the sequences of 160nt between-376 and-217 nt are essential to its IRES function.The FOSB IRES activity can be stimulated in Bel-7402 treated with paclitaxel or cis-platinum,especially under the stimulating of paclitaxel.All these findings provide a new thought and direction for the study of FOSB in cancer prevention and treatment.

关 键 词：小鼠骨肉瘤病毒致癌基因同族体B 内部核糖体进入位点 5′非翻译区 荧光素酶 

分 类 号：Q522[生物学—生物化学]