多花黄精多糖对STZ诱导的Ⅰ型糖尿病小鼠影响作用研究  

Hypoglycemic Effects of Polysaccharide of Polygonatum Cyrtonema Hua on Type1 Diabetic Mice Induced by STZ

作　　者：王秋丽 童小慧 李小东 韩荣春[1] 彭代银[1] 俞年军[1] WANG Qiuli;TONG Xiaohui;LI Xiaodong;HAN Rongchun;PENG Daiyin;YU Nianjun(Institute of Resource Protection and Development of Traditional Chinese Medicine,Anhui Academy of Traditional Chinese Medicine,Anhui University of Chinese Medicine,Hefei 231200,China;College of Integrated Chinese and Western Medicine,Anhui University of Chinese Medicine,Hefei 230012,China)

机构地区：[1]安徽中医药大学安徽省中医药科学院中药资源保护与开发研究所,安徽合肥231200 [2]安徽中医药大学中西医结合学院,安徽合肥230012

出　　处：《云南中医学院学报》2019年第1期1-7,共7页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家重点研发计划（2017YFC1701602）;安徽省重点研究与开发计划项（1804b06020356）;安徽中医药大学自然重点项目（2019zrzd02)

摘　　要：目的研究多花黄精多糖对Ⅰ型糖尿病小鼠血糖、存活率及肝保护等的影响。方法采用连续腹腔注射链脲佐菌素(STZ),建立雌性C57BL/6c小鼠Ⅰ型糖尿病模型。实验分四组:空白对照组、模型组、多花黄精多糖低剂量组(450 zmg·kg^-1)和高剂量组(900 mg·kg^-1)。实验中检测各组小鼠存活率、体重以及血糖变化。实验结束后,分离小鼠肝脏,采用HE染色法评价肝脏病理变化,实时定量PCR(qPCR)法检测肝脏中白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、胰岛素受体底物1(IRS-1)mRNA水平。结果雌性C57BL/6c小鼠连续5天腹腔注射STZ,一周后可使血糖明显升高。在高血糖状态下,模型组小鼠死亡率急剧上升,多花黄精多糖高剂量显著提高模型小鼠的存活率;多花黄精多糖低、高剂量可显著增加模型小鼠体重;同时,多花黄精多糖高剂量有一定程度降低血糖的作用。HE染色结果显示,模型小鼠肝脏病变明显:出现大量的单核炎症细胞浸润,肝细胞发生变性明显。并且,肝细胞结构紊乱。而多花黄精多糖高剂量能够改善模型小鼠肝脏炎症浸润、肝细胞变性以及肝脏结构紊乱等病变。qPCR结果显示多花黄精多糖能显著降低糖尿病肝脏中IL-6,IL-1βmRNA的水平,显著升高IRS-1 mRNA的水平。结论多花黄精多糖显著降低Ⅰ型糖尿病小鼠的死亡率,可能与其改善小鼠体重、降低血糖、肝保护等相关。Objective This experiment is intended to explore effects of polysaccharide of P.cyrtonema Hua on glycemia,survival rates and liver protection in streptozotocin(STZ)inducedⅠdiabetes mice.Methods Type I diabetes was induced in female C57BL/6c mice by continuous intraperitoneal injection of STZ(70 mg·kg^-1).Mice was randomly divided into four groups:control,model,polysaccharide of P.cyrtonema Hua of low-dose(450 mg·kg^-1)and high group(900 mg·kg^-1).One week after the first injection of STZ,mice in each group were given corresponding drugs by gavage,with mice in control and model group dosaged with water.The survival rate,body weight and blood glucose of mice were measured.at the end of the experiment,liver was isolated and the histological changes were evaluated by HE staining.Gene expressions of IL-6,IL-1β,IRS-1 in the liver were detected by qPCR.Results Female C57BL/6c mice’s blood glucose rapidly increased one week after being injected with STZ(70 mg·kg^-1)for 5 consecutive days.Hyperglycemia sharply increased the mortality of mice,which was significantly reversed by high dose of polysaccharide of P.cyrtonema Hua.Besides,low and high dose of polysaccharide of P.cyrtonema Hua(450-900 mg·kg^-1)significantly increased the body weight of model mice to some extent.Polysaccharide of P.cyrtonema Hua slightly but significantly reversed hyperglycemia in model mice.Histologically,liver damage of model mice was demonstrated with mononuclear inflammatory cells infiltration,hepatocyte cytoplasm and nuclear pyknosis.High dose of polysaccharide of P.cyrtonema Hua can ameliorate these pathological changes such as inflammation,liver cell degeneration and liver structural loss of model mice.qPCR results confirmed that,compared with the model group,polysaccharide of P.cyrtonema Hua could significantly reduce the levels of IL-6 and IL-1 beta mRNA and increase the levels of IRS-1 mRNA in the liver.Conclusion Polysaccharide of P.cyrtonema Hua significantly reduced the acute mortality of T1DM mice,which may be accounted by i

关 键 词：多花黄精多糖 Ⅰ型糖尿病 存活率 肝保护 

分 类 号：R285.5[医药卫生—中药学]