复方参鹿颗粒调节相对低危骨髓增生异常综合征患者TGF-β1介导的Treg/Th17细胞变化的临床研究  

Clinic Observation on Effect of Fufang Shenlu Granule on Treg/Th17 Cells and Related Transcription Factors of the Patients with Lower Risk Myelodysplastic Syndromes by Regulating TGF-β1

作　　者：陆颖佳[1] 陈珮[1] 邱仲川[1] 赵琳[1] 胡晓莹[1] 朱小勤[1] 瞿玮颖 封舟[1] 黄中迪[1] LU Yingjia;CHEN Pei;QIU Zhongchuan;ZHAO Lin;HU Xiaoying;ZHU Xiaoqin;QU Weiying;FENG Zhou;HUANG Zhongdi(Shuguang Hospital Shanghai University of Traditional Chinese Medcine,Shanghai 200021,China)

机构地区：[1]上海中医药大学附属曙光医院血液科

出　　处：《云南中医学院学报》2019年第1期33-37,共5页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金资（81403233）;上海市卫生和计划生育委员会中医药科研专项课题（2016LP045）

摘　　要：目的探讨复方参鹿颗粒对相对低危MDS患者治疗前后外周血TGF-β1、IL-2、IL-6、Treg细胞、Th17细胞、相关转录因子及比值的影响。方法将40例相对低危MDS患者随机分成中药组和对照组,中药组予复方参鹿颗粒联合西药治疗,对照组单用西药治疗。2组均6个月为1个疗程,共治疗1个疗程,观察外周血TGF-β1、IL-2、IL-6、Treg细胞、Th17细胞、相关转录因子及比值变化。结果治疗后中药组TGF-β1、IL-2表达上升,IL-6表达下降;TGF-β1、IL-2/IL-6比率均高于对照组,IL-6表达低于对照组,均有明显差异(P<0.05)。Treg细胞表达、Treg/Th17比值均高于对照组,具有统计学意义(P<0.05)。治疗后中药组Foxp3mRNA表达、Foxp3/RoRγt比值检测均高于对照组,有明显差异(P<0.05)。结论复方参鹿颗粒可以改善相对低危MDS患者TGF-β1、IL-6、IL-2/IL-6比率、Treg细胞、Treg/Th17比率。提示复方参鹿颗粒改善相对低危MDS患者的造血功能可能通过调整TGF-β1、IL-2/IL-6来调控Treg/Th17比率,进一步改善T细胞免疫功能有关。Objective To observe the Effect of Fufang Shenlu Granule on TGF-β1,IL-2,IL-6,Treg cells,Th17 cells and related transcription factors of the patients with lower risk myelodysplastic syndromes.Methods 40 patients with lower-risk MDS were randomly divided into treatment group and controlled group.Treatment group was treated with Fufang Shenlu Granule combined with conventional medicine therapy,while the control group was only treated with conventional medicine therapy.Two groups were treated with one course(one course is 6 months).We examined TGF-β1,IL-2,IL-6,Treg cells,Th17 cells,related transcription factors and related ratio after treatment.Results After treatment,TGF-β1,IL-2/IL-6,Treg cells and Treg/Th17 cells,Foxp3 mRNA and the ratio of peripheral blood Foxp3/RoRγt mRNA of treatment group were higher than those of controlled group(P<0.05).IL-6 was lower than controlled group(P<0.05).Conclusion Fufang Shenlu Granule can abjust Treg cells,the balance of Treg/Th17 cells and Foxp3 mRNA and Foxp3/RoRγt mRNA the patients with lower risk myelodysplastic syndromes by TGF-β1,IL-6,and IL-2/IL-6.The mechanism on the treatment efficiency of Fufang Shenlu Granule is associated with Immune dysregulation.

关 键 词：骨髓增生异常综合征 复方参鹿颗粒 TGF-Β1 IL-2 IL-6 TREG细胞 

分 类 号：R259[医药卫生—中西医结合]