高粱TCP基因家族全基因组鉴定及表达分析  

Genome-Wide Identification and Expression Analysis of TCP Gene Family in Sorghum bicolor L.

作　　者：郑玲 白雪婷 李会云 ZHENG Ling;BAI Xueting;LI Huiyun(College of Life Science,Luoyang Normal University,Luoyang 471934,China)

机构地区：[1]洛阳师范学院生命科学学院

出　　处：《河南农业科学》2019年第10期30-36,共7页Journal of Henan Agricultural Sciences

基　　金：河南省科技攻关计划项目(192102110035)

摘　　要：为了研究高粱TCP(Teosinte branched 1/cycloidea/proliferating cell factors)基因家族在生长发育和逆境胁迫中的作用,利用生物信息学方法对高粱TCP基因家族成员进行鉴定,并对其进行染色体定位、保守结构域、进化树和表达模式分析。结果表明,共鉴定出27个高粱TCP基因,其在9条染色体上呈不均匀分布,并在3、6、9号等染色体上存在明显的基因簇。进化树分析显示,高粱TCP蛋白可被分为ClassⅠ和ClassⅡ2大类,而ClassⅡ类又可分为CYC/TB1和CIN 2个亚类,ClassⅠ包含10个TCP蛋白,CYC/TB1包含3个TCP蛋白,CIN包含14个TCP蛋白。27个高粱TCP蛋白均有非典型的bHLH(Basic-helix-loop-helix)保守区,其中2个有R结构域。表达模式分析表明,大部分高粱TCP基因在种子、胚、雌蕊、早期花序中表达;PEG处理下,高粱根中大部分TCP基因表达量升高,ABA处理嫩枝中个别基因表达量升高,推测上调表达的高粱TCP基因可能在干旱胁迫响应过程中起重要作用。In order to study the function of TCP(Teosinte-branched 1/cycloidea/proliferating cell factors)gene family of Sorghum bicolor L.in growth and response to adversity stress,the SbTCP genes were identified by bioinformatic analysis,and their conserved domain,phylogenetic relationship,chromosome location,conserved domain and expression pattern were analyzed.The results showed that a total of 27 SbTCP genes were identified,they were unevenly distributed across 9 chromosomes,and there were gene clusters on 3,6,9 chromosomes.All the SbTCP proteins could be divided into two groups,ClassⅠand ClassⅡ,and ClassⅡcould be divided into two subgroups,CIN and CYC/TB1.ClassⅠcontained 10 SbTCP proteins,CYC/TB1 contained 3 SbTCP proteins,and CIN contained 14 SbTCP proteins.Twenty-seven SbTCP proteins all had atypical bHLH(Basic-helix-loop-helix)conserved region,and two SbTCP proteins had R domain.Transcriptome analysis showed that most of SbTCP genes expressed in seed,embryo,pistil and early inflorescence;the expression level of most of SbTCP genes increased in roots under PEG treatment,and the expression level of several SbTCP genes increased in shoots under ABA treatment,which suggested that SbTCP genes might play important role in response to drought stress.

关 键 词：高粱 TCP基因 全基因组鉴定 表达分析 ABA处理 PEG处理 

分 类 号：S514[农业科学—作物学]