蛴螬提取物对实验性兔视网膜静脉阻塞新生血管的影响  

Effect of White Grub Extract on Neovascularization of Experimental Rabbit Retinal Vein Obstruction

作　　者：蒋鹏飞[1,2] 梁凯霞 彭俊[2,3] 彭清华 JIANG Pengfei;LIANG Kaixia;PENG Jun;PENG Qinghua(Hunan University of Chinese Medicine,Changsha Hunan China 410208;The Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Eye,Ear,Nose and Throat Diseases in Hunan Province,Changsha Hunan China 410007;The First Affiliated Hospital to Hunan University of Chinese Medicine,Changsha Hunan China 410208)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]中医药防治眼耳鼻咽喉疾病湖南省重点实验室,湖南长沙410208 [3]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中医学报》2019年第10期2144-2147,共4页Acta Chinese Medicine

基　　金：国家自然科学基金面上资助项目(30772824,81574031,81804150);湖南省重点实验室建设项目(2017TP1018);长沙市科技计划项目(K1501014-31,kc1704005)

摘　　要：目的:分析蛴螬提取物对实验性兔视网膜静脉阻塞的治疗作用及其机制。方法:40只兔随机分为空白组、模型组、复方血栓通组和蛴螬组,每组10只,除空白组外,其余3组均用光化学动力法建立视网膜静脉阻塞(retinal vein occlusion,RVO)动物模型,于给药后1周、3周行眼底荧光素血管造影(fundus fluorescein angiography,FFA)检查、HE染色、免疫组化检测视网膜肝细胞生长因子(hepatocyte growth factor,HGF)表达。结果:HE染色结果显示:蛴螬组视网膜各层结构病理变化均轻于模型组、复方血栓通组。HGF免疫组化结果显示:造模后1周,蛴螬组与复方血栓通组HGF表达均降低,与模型组比较,差异有统计学意义(P<0.05);造模后3周,蛴螬组与复方血栓通组较1周前均有所降低,蛴螬组造模后3周与造模后1周比较,差异有统计学意义(P<0.05)。结论:蛴螬提取物能促进实验性兔RVO出血的吸收,维持视网膜各层正常的结构,降低实验性兔RVO模型视网膜HGF的表达,这可能是蛴螬抑制RVO后CNV形成的可能机制。Objective:To evaluate the therapeutic effect and mechanism of white grub extract on experimental rabbit retinal vein occlusion.Methods:Forty rabbits were randomly divided into the blank group,the model group,the compound Xueshuantong group and the white grub group.Each group of 10 rabbits,except for the blank group,was established by photochemical kinetic method to establish retinal vein occlusion.In the animal model of RVO,the expression of hepatocyte growth factor(HGF) was detected by fundus fluorescein angiography(FFA),HE staining and immunohistochemistry at 1 week and 3 weeks after administration.Results:The results of HE staining suggests that the pathological changes of the retina layers in the sputum group were lighter than those in the model group and the compound thrombus group.The results of HGF immunohistochemistry showed that the expression of HGF in the sputum group and the compound Xueshuantong group decreased at 1 week after model establishment,and the difference was statistically significant(P<0.05).After 3 weeks of modeling,the sputum group and The compound Xueshuantong group was lower than that before 1 week,and the difference was statistically significant at 3 weeks after model establishment and 1 week after model establishment(P<0.05).Conclusion:The white grub extract can promote the absorption of RVO hemorrhage in experimental rabbits,maintain the normal structure of the retina and reduce the expression of HGF in the experimental rabbit RVO model,which may be the possible mechanism of CNV formation after RVO inhibition.

关 键 词：视网膜静脉阻塞 蛴螬提取物 视网膜肝细胞生长因子 兔 

分 类 号：R285.5[医药卫生—中药学]